dc.contributor.author |
Meade, SO |
en |
dc.contributor.author |
Chen, MY |
en |
dc.contributor.author |
Sailor, MJ |
en |
dc.contributor.author |
Miskelly, Gordon |
en |
dc.date.accessioned |
2012-01-31T20:25:14Z |
en |
dc.date.issued |
2009 |
en |
dc.identifier.citation |
Analytical Chemistry 81(7):2618-2625 2009 |
en |
dc.identifier.issn |
0003-2700 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/10830 |
en |
dc.description.abstract |
A particle-based multiplexed DNA assay based on encoded porous SiO2 photonic crystal disks is demonstrated. A “spectral barcode” is generated by electrochemical etch of a single-crystal silicon wafer using a programmed current−time waveform. A lithographic procedure is used to isolate cylindrical microparticles 25 μm in diameter and 10 μm thick, which are then oxidized, modified with a silane linker, and conjugated to various amino-functionalized oligonucleotide probes via cyanuric chloride. It is shown that the particles can be decoded based on their reflectivity spectra and that a multiple analyte assay can be performed in a single sample with a modified fluorescence microscope. The homogeneity of the reflectivity and fluorescence spectra, both within and across the microparticles, is also reported. |
en |
dc.publisher |
American Chemical Society |
en |
dc.relation.ispartofseries |
Analytical Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0003-2700/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Multiplexed DNA detection using spectrally encoded Porous SiO photonic crystal particles |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1021/ac802538x |
en |
pubs.issue |
7 |
en |
pubs.begin-page |
2618 |
en |
pubs.volume |
81 |
en |
dc.rights.holder |
Copyright: American Chemical Society |
en |
dc.identifier.pmid |
19271746 |
en |
pubs.end-page |
2625 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
93362 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Chemistry |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
19271746 |
en |