dc.contributor.author |
Williman, Jonathan |
en |
dc.contributor.author |
Slobbe, L |
en |
dc.contributor.author |
Buchan, G |
en |
dc.contributor.author |
Baird, MA |
en |
dc.date.accessioned |
2012-02-09T02:29:07Z |
en |
dc.date.issued |
2006 |
en |
dc.identifier.citation |
VACCINE 24(21):4471-4474 22 May 2006 |
en |
dc.identifier.issn |
0264-410X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/11032 |
en |
dc.description.abstract |
Unlike intramuscular injection, gene gun delivery of DNA drives a strong type 2 response. In an effort to counter this, we have genetically fused the type 1 cytokines, IL-12 and IL-23, to the hemagglutinin (HA) gene from influenza APR/8/34, and delivered these DNA constructs to Balb/c mice. Gene gun delivery of the HA gene was able to induce antibody production by all vaccinated mice. Linking of IL-12 caused almost complete suppression of immune responses whereas mice vaccinated with IL-23HA showed long-lived IgG1 antibody levels. Splenocytes from IL-23HA vaccinated mice also tended to produce more IL-5 and IFNγ after restimulation in vitro than splenocytes from HA vaccinated mice. While codelivery of IL-23 did not change the type of immune response it may increase its longevity following vaccination. |
en |
dc.publisher |
Elsevier Ltd. |
en |
dc.relation.ispartofseries |
Vaccine |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
The use of Th1 cytokines, IL-12 and IL-23, to modulate the immune response raised to a DNA vaccine delivered by gene gun. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.vaccine.2005.08.011 |
en |
pubs.issue |
21 |
en |
pubs.begin-page |
4471 |
en |
pubs.volume |
24 |
en |
dc.rights.holder |
Copyright: Elsevier Ltd. |
en |
dc.identifier.pmid |
16140432 |
en |
pubs.end-page |
4474 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
102809 |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
16140432 |
en |