dc.contributor.author |
Jarrett, RG |
en |
dc.contributor.author |
Ruggiero, Katya |
en |
dc.date.accessioned |
2012-02-22T23:15:52Z |
en |
dc.date.issued |
2008 |
en |
dc.identifier.citation |
Biometrics 64(1):208-216 Mar 2008 |
en |
dc.identifier.issn |
0006-341X |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/11622 |
en |
dc.description.abstract |
Gene expression microarrayexp eriments are intrinsicallyt wo-phase experiments. Messenger RNA (mRNA), required for the microarrayexp eriment, must first be derived from plants or animals that are exposed to a set of treatments in a previous experiment (Phase 1). The mRNA is then used in the subsequent laboratory-based microarray experiment (Phase 2) from which gene expression is measured and ultimatelyanaly zed. We show that obtaining a valid test for the effects of treatments on gene expression depends on the design of both the Phase 1 and Phase 2 experiments. Examples show that the multiple dyeswap design at Phase 2 is more robust than the alternating loop design in the absence of prior knowledge of the relative size of variation in the Phase 1 and Phase 2 experiments. |
en |
dc.publisher |
Wiley-Blackwell Publishing Ltd. |
en |
dc.relation.ispartofseries |
Biometrics |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0006-341X/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Design and analysis of two-phase experiments for gene expression microarray experiments |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/j.1541-0420.2007.00835.x |
en |
pubs.begin-page |
208 |
en |
pubs.volume |
64 |
en |
dc.rights.holder |
Copyright: Wiley-Blackwell Publishing Ltd. |
en |
dc.identifier.pmid |
17651456 |
en |
pubs.end-page |
216 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
91691 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Statistics |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
17651456 |
en |