dc.contributor.author |
Yu, Tzu-Ying |
en |
dc.contributor.author |
Acosta Etchebarne, Monica |
en |
dc.contributor.author |
Ready, S |
en |
dc.contributor.author |
Cheong, YL |
en |
dc.contributor.author |
Kalloniatis, M |
en |
dc.date.accessioned |
2012-02-22T23:56:29Z |
en |
dc.date.issued |
2007 |
en |
dc.identifier.citation |
J Neurochem 103(2):714-724 Oct 2007 |
en |
dc.identifier.issn |
0022-3042 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/11646 |
en |
dc.description.abstract |
Light exposure induces retinal photoreceptor degeneration and retinal remodeling in both the normal rat retina and in animal models of retinal degeneration. Although cation entry is one of the triggers leading to apoptosis, it is unclear if this event occurs in isolation, or whether a number of pathways lead to photoreceptor apoptosis following light exposure. Following light exposure, we investigated the characteristics of cation entry, apoptotic markers [using terminal deoxynucleotidyl transferase (EC 2.7.7.31) dUTP nick-end labeling (TUNEL) labeling] and metabolic properties of retina from Sprague–Dawley (SD) rats and a rat model of retinitis pigmentosa [proline-23-histidine (P23H) rat]. Assessment of cation channel permeability using agmatine (AGB) labeling showed that excessive cation gating accompanied the series of anomalies that occur prior to photoreceptor loss. Increased AGB labeling in photoreceptors was seen in parallel with the appearance of apoptotic photoreceptors detected by TUNEL labeling with only a smaller proportion of cells colocalizing both markers. However, SD and P23H retinal photoreceptors differed in the amounts and colocalization of AGB gating and TUNEL labeling as a function of light exposure. Finally, reduced retinal lactate dehydrogenase levels were found in SD and P23H rat retinas after a 24-h light exposure period. Short-term (2 h) exposure of the P23H rat retina caused an increase in lactate dehydrogenase activity suggesting increased metabolic demand. These results suggest that energy availability may be exacerbated during the early stages of light exposure in susceptible retinas. Also, the concomitant observation of increased ion gating and TUNEL labeling suggest the existence of at least two possible mechanisms in light-damaged retinas in both SD and the P23H rat retina. |
en |
dc.publisher |
International Society for Neurochemistry |
en |
dc.relation.ispartofseries |
Journal of Neurochemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
Details obtained from http://www.sherpa.ac.uk/romeo/issn/0022-3042/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Light exposure causes functional changes in the retina: increased photoreceptors cation channel permeability, photoreceptor apoptosis and altered retinal metabolic function |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/j.1471-4159.2007.04766.x |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
714 |
en |
pubs.volume |
103 |
en |
dc.rights.holder |
Copyright: International Society for Neurochemistry |
en |
dc.identifier.pmid |
17623037 |
en |
pubs.end-page |
724 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
79962 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Optometry and Vision Science |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
17623037 |
en |