dc.contributor.author |
Sarojini Amma, Vijayalekshmi |
en |
dc.contributor.author |
Rao, RB |
en |
dc.contributor.author |
Ragothama, S |
en |
dc.contributor.author |
Balaram, P |
en |
dc.date.accessioned |
2012-02-23T00:20:30Z |
en |
dc.date.issued |
2010 |
en |
dc.identifier.citation |
Journal of Peptide Science 16(8):430-436 Aug 2010 |
en |
dc.identifier.issn |
1075-2617 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/11684 |
en |
dc.description.abstract |
The solution conformation of a designed tetradecapeptide Boc-Val-Ala-Leu-Dpg-Val-Ala-Leu-Val-Ala-Leu-Dpg-Val-Ala-Leu-OMe (Dpg-14) containing two di-n-propyl glycine (Dpg) residues has been investigated by ^1H NMR and circular dichroism in organic solvents. The peptide aggregates formed at a concentration of 3 mM in the apolar solvent CDCl3 were broken by the addition of 12% v/v of the more polar solvent DMSO-d6. Successive NiH Ni+1H NOEs observed over the entire length of the sequence in this solvent mixture together with the observation of several characteristic medium-range NOEs support a major population of continuous helical conformations for Dpg-14. Majority of the observed coupling constants (3JNHCαH) also support φ values in the helical conformation. Circular dichroism spectra recorded in methanol and propan-2-ol give further support in favor of helical conformation for Dpg-14 and the stability of the helix at higher temperature. |
en |
dc.publisher |
John Wiley & Sons Ltd. |
en |
dc.relation.ispartofseries |
Journal of Peptide Science |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from:
http://www.sherpa.ac.uk/romeo/issn/1075-2617/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Solution conformation of a tetradecapeptide stabilized by two di-n-propyl glycine residues |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1002/psc.1259 |
en |
pubs.begin-page |
430 |
en |
pubs.volume |
16 |
en |
dc.rights.holder |
Copyright: John Wiley & Sons Ltd. |
en |
dc.identifier.pmid |
20623490 |
en |
pubs.end-page |
436 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
102704 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Chemistry |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
20623490 |
en |