The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit Softening

Show simple item record Tacken, E en Ireland, H en Gunaseelan, K en Karunairetnam, S en Wang, D en Schultz, K en Bowen, J en Atkinson, RG en Johnston, JW en Putterill, Joanna en Hellens, RP en Schaffer, Robert en 2012-02-23T00:31:43Z en 2010 en
dc.identifier.citation Plant Physiology 153(1):294-305 May 2010 en
dc.identifier.issn 0032-0889 en
dc.identifier.uri en
dc.description.abstract Fruit softening in apple (Malus 3 domestica) is associated with an increase in the ripening hormone ethylene. Here, we show that in cv Royal Gala apples that have the ethylene biosynthetic gene ACC OXIDASE1 suppressed, a cold treatment preconditions the apples to soften independently of added ethylene. When a cold treatment is followed by an ethylene treatment, a more rapid softening occurs than in apples that have not had a cold treatment. Apple fruit softening has been associated with the increase in the expression of cell wall hydrolase genes. One such gene, POLYGALACTURONASE1 (PG1), increases in expression both with ethylene and following a cold treatment. Transcriptional regulation of PG1 through the ethylene pathway is likely to be through an ETHYLENE-INSENSITIVE3-like transcription factor, which increases in expression during apple fruit development and transactivates the PG1 promoter in transient assays in the presence of ethylene. A cold-related gene that resembles a COLD BINDING FACTOR (CBF) class of gene also transactivates the PG1 promoter. The transactivation by the CBF-like gene is greatly enhanced by the addition of exogenous ethylene. These observations give a possible molecular mechanism for the cold-and ethylene-regulated control of fruit softening and suggest that either these two pathways act independently and synergistically with each other or cold enhances the ethylene response such that background levels of ethylene in the ethylene-suppressed apples is sufficient to induce fruit softening in apples. en
dc.language EN en
dc.publisher American Society of Plant Biologists en
dc.relation.ispartofseries Plant Physiology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: en
dc.rights.uri en
dc.subject WATER-DEFICIT en
dc.subject ARABIDOPSIS en
dc.subject EXPRESSION en
dc.subject FAMILY en
dc.subject TOMATO en
dc.subject BIOSYNTHESIS en
dc.subject RESPONSES en
dc.title The Role of Ethylene and Cold Temperature in the Regulation of the Apple POLYGALACTURONASE1 Gene and Fruit Softening en
dc.type Journal Article en
dc.identifier.doi 10.1104/pp.109.151092 en
pubs.begin-page 294 en
pubs.volume 153 en
dc.rights.holder Copyright: American Society of Plant Biologists en
dc.identifier.pmid 20237022 en
pubs.end-page 305 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 162903 en Science en Biological Sciences en
pubs.record-created-at-source-date 2010-12-01 en
pubs.dimensions-id 20237022 en

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