dc.contributor.author |
Wang, IY |
en |
dc.contributor.author |
Bai, Y |
en |
dc.contributor.author |
Sanderson, MJ |
en |
dc.contributor.author |
Sneyd, A |
en |
dc.coverage.spatial |
United States |
en |
dc.date.accessioned |
2012-02-27T00:25:34Z |
en |
dc.date.issued |
2010-04-07 |
en |
dc.identifier.citation |
Biophysical Journal 98(7):1170-1181 07 Apr 2010 |
en |
dc.identifier.issn |
0006-3495 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/12064 |
en |
dc.description.abstract |
Airway hyperresponsiveness is a major characteristic of asthma and is generally ascribed to excessive airway narrowing associated with the contraction of airway smooth muscle cells (ASMCs). ASMC contraction is initiated by a rise in intracellular calcium concentration ([Ca(2+)](i)), observed as oscillatory Ca(2+) waves that can be induced by either agonist or high extracellular K(+) (KCl). In this work, we present a model of oscillatory Ca(2+) waves based on experimental data that incorporate both the inositol trisphosphate receptor and the ryanodine receptor. We then combined this Ca(2+) model and our modified actin-myosin cross-bridge model to investigate the role and contribution of oscillatory Ca(2+) waves to contractile force generation in mouse ASMCs. The model predicts that: 1), the difference in behavior of agonist- and KCl-induced Ca(2+) waves results principally from the fact that the sarcoplasmic reticulum is depleted during agonist-induced oscillations, but is overfilled during KCl-induced oscillations; 2), regardless of the order in which agonist and KCl are added into the cell, the resulting [Ca(2+)](i) oscillations will always be the short-period, agonist-induced-like oscillations; and 3), both the inositol trisphosphate receptor and the ryanodine receptor densities are higher toward one end of the cell. In addition, our results indicate that oscillatory Ca(2+) waves generate less contraction than whole-cell Ca(2+) oscillations induced by the same agonist concentration. This is due to the spatial inhomogeneity of the receptor distributions. |
en |
dc.language |
eng |
en |
dc.publisher |
Cell Press |
en |
dc.relation.ispartofseries |
Biophysical Journal |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0006-3495/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Animals |
en |
dc.subject |
Biophysics |
en |
dc.subject |
Calcium |
en |
dc.subject |
Inositol 1,4,5-Trisphosphate Receptors |
en |
dc.subject |
Membrane Potentials |
en |
dc.subject |
Mice |
en |
dc.subject |
Models, Theoretical |
en |
dc.subject |
Myocytes, Smooth Muscle |
en |
dc.subject |
Oocytes |
en |
dc.subject |
Oscillometry |
en |
dc.subject |
Potassium Chloride |
en |
dc.subject |
Ryanodine Receptor Calcium Release Channel |
en |
dc.subject |
Stochastic Processes |
en |
dc.subject |
Trachea |
en |
dc.subject |
Xenopus |
en |
dc.title |
A mathematical analysis of agonist- and KCl-induced Ca(2+) oscillations in mouse airway smooth muscle cells. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.bpj.2009.12.4273 |
en |
pubs.issue |
7 |
en |
pubs.begin-page |
1170 |
en |
pubs.volume |
98 |
en |
dc.rights.holder |
Copyright: Cell Press |
en |
dc.identifier.pmid |
20371316 |
en |
pubs.end-page |
1181 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
205877 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Mathematics |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1542-0086 |
en |
dc.identifier.pii |
S0006-3495(09)06086-X |
en |
pubs.record-created-at-source-date |
2012-02-22 |
en |
pubs.dimensions-id |
20371316 |
en |