Investigations on the cell walls of commelinid monocotyledons

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dc.contributor.advisor Associate Professor Philip Harris en
dc.contributor.advisor Professor Peter Lovell en Trethewey, Jason Allan Karl en 2007-08-04T09:56:38Z en 2007-08-04T09:56:38Z en 2004 en
dc.identifier.citation Thesis (PhD--Biological Sciences)--University of Auckland, 2004. en
dc.identifier.uri en
dc.description Restricted Item. Print thesis available in the University of Auckland Library or may be available through Interlibrary Loan. en
dc.description.abstract Four topics concerning the cell walls of commelinid monocotyledons were investigated. First, the distribution of (1→3),(1→4)-β-glucans and (1→3)-β-glucans in the walls of different cell types in vegetative organs of barley (Hordeum vulgare) was determined using immunogold labelling with monoclonal antibodies. All walls were labelled with the antibody to (1→3),(1→4)-β-glucans, except those of the outer root cap. Two types of labelling distribution were found over primary walls: only adjacent to the plasma membrane, and throughout the wall and middle lamella. Small amounts of labelling occurred with the (1→3)-β-glucan antibody, mostly over plasmodesmata. Both antibodies labelled cell plates. Second, changes in location and density of (1→3),(1→4)-β-glucans in specific walls of barley coleoptiles during development were investigated by immunogold labelling and analysed quantitatively. In non-lignified walls from 2.5- to 5-days after germination, three different patterns of change were recognized. In most walls, there was an increasing amount of label located evenly throughout the walls (Type A pattern). Some walls showed a decrease in labelling density in the area beneath the cuticle but an increase in the area adjacent to the plasma membrane (Type B), whereas others showed decreasing amounts of label located evenly throughout the walls (Type C). In lignified walls (Type D) the label was confined mainly to the compound middle lamella. Third, the location of (1→3),(1→4)-ß-glucans in walls of vegetative organs from 9 families of the Poales (s.l.) was determined by immunogold labelling. Three types of wall-labelling pattern were identified depending on the labelling of specific walls. Type 1 (Poaceae and Flagellariaceae) had the heaviest labelling of non-lignified walls and Type 2 (Restionaceae and Xyridaceae) the heaviest labelling of lignified walls. Type 3 (Cyperaceae and Juncaceae) had only very light labelling of all walls. Walls of the Typhaceae, Sparganiaceae, and Bromeliaceae were unlabelled. Fourth, the ferulic and p-coumaric acids ester-linked to non-lignified primary walls from 11 families of commelinid monocotyledons were quantified. These acids were present in the walls of all species, with trans-ferulic acid the most abundant. ‘Driselase’ digestion and mild acid hydrolysis of isolated walls released ferulolyl oligosaccharides with structures that indicated the ferulic acid was ester-linked to glucuronoarabinoxylans. en
dc.format Scanned from print thesis en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA1503634 en
dc.rights Whole document restricted. Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri en
dc.title Investigations on the cell walls of commelinid monocotyledons en
dc.type Thesis en Biological Sciences en The University of Auckland en Doctoral en PhD en
dc.subject.marsden Fields of Research::270000 Biological Sciences en
dc.rights.holder Copyright: The author en
pubs.local.anzsrc 06 - Biological Sciences en
dc.rights.accessrights en Faculty of Science en

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