Conjugation of oligosaccharides with casein

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dc.contributor.advisor Melton, Laurence en
dc.contributor.author Pan, Geoffrey Ge en
dc.date.accessioned 2007-08-04T11:31:17Z en
dc.date.available 2007-08-04T11:31:17Z en
dc.date.issued 2005 en
dc.identifier THESIS 05-189 en
dc.identifier.citation Thesis (PhD--Food Science)--University of Auckland, 2005 en
dc.identifier.uri http://hdl.handle.net/2292/1245 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract In this study, sialyl oligosaccharides (SOs) were isolated from bovine colostrum using an ion exchange method. The method was modified by using ammonium bicarbonate as the eluent which can be readily removed by cation exchange resin. SOs were characterized using high performance liquid chromatography-electrospray ionization-mass spectrometry (HPLC-ESI-MS), and the present study found that the ESI mass spectral patterns of α-2-3 and α-2-6 sialyl linkages were different. The lactonisation of disialyl lactose (DSL) was investigated. The 1H and 13C NMR spectra of DSL dilactone are reported for the first time Non-enzymatic browning between lactose and sodium caseinate was studied at different relative humidities (RHs) using a variety of analytical methods such as furosine, loss of lysine UV absorbance, fluorescence, and loss of lactose. All the analytical methods showed that the maximal conjugation rate occurred at intermediate RH. However, some discrepancies existed between the results of different methods. The results also showed that the degradation of Amadori product increased with RH. It is the first time that the conjugation of sialyl lactose with casein through Maillard reaction has been studied. An HPLC-UV-MS method was established to monitor the α-dicarbonyls generated during the lactose-casein conjugation. Bioassay results showed that both lactose-casein and sialyl lactose-casein conjugates have specific inhibition effects on S. typhimurium binding to cells derived from human colonic adenocarcinoma. Based on the sialic acid content, sialyl lactose-casein conjugate exerts the effect at much lower concentration than free sialyl lactose. This could be attributed to a polyvalent effect. en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA99148859114002091 en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Conjugation of oligosaccharides with casein en
dc.type Thesis en
thesis.degree.discipline Food Science en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.rights.holder Copyright: The author en


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