Analysis of expressed sequence tags from Actinidia: Applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening

Show simple item record Crowhurst, RN en Gleave, AP en MacRae, EA en Ampomah-Dwamena, C en Atkinson, RG en Beuning, LL en Bulley, SM en Chagne, D en Marsh, KB en Matich, AJ en Montefiori, M en Newcomb, Richard en Schaffer, Robert en Usadel, B en Allan, Andrew en Boldingh, HL en Bowen, JH en Davy, MW en Eckloff, R en Ferguson, AR en Fraser, LG en Gera, E en Hellens, RP en Janssen, BJ en Klages, K en Lo, KR en MacDiarmid, Robin en Nain, B en McNeilage, MA en Rassam, M en Richardson, AC en Rikkerink, EHA en Ross, GS en Schroder, R en Snowden, Kimberley en Souleyre, EJF en Templeton, Matthew en Walton, EF en Wang, D en Wang, MY en Wang, YY en Wood, M en Wu, RM en Yauk, YK en Laing, WA en 2012-03-04T19:37:13Z en 2008 en
dc.identifier.citation BMC GENOMICS 9:1-26 Article number 351 2008 en
dc.identifier.issn 1471-2164 en
dc.identifier.uri en
dc.description.abstract Background: Kiwifruit (Actinidia spp.) are a relatively new, but economically important crop grown in many different parts of the world. Commercial success is driven by the development of new cultivars with novel consumer traits including flavor, appearance, healthful components and convenience. To increase our understanding of the genetic diversity and gene-based control of these key traits in Actinidia, we have produced a collection of 132,577 expressed sequence tags (ESTs). Results: The ESTs were derived mainly from four Actinidia species (A. chinensis, A. deliciosa, A. arguta and A. eriantha) and fell into 41,858 non redundant clusters (18,070 tentative consensus sequences and 23,788 EST singletons). Analysis of flavor and fragrance-related gene families (acyltransferases and carboxylesterases) and pathways (terpenoid biosynthesis) is presented in comparison with a chemical analysis of the compounds present in Actinidia including esters, acids, alcohols and terpenes. ESTs are identified for most genes in color pathways controlling chlorophyll degradation and carotenoid biosynthesis. In the health area, data are presented on the ESTs involved in ascorbic acid and quinic acid biosynthesis showing not only that genes for many of the steps in these pathways are represented in the database, but that genes encoding some critical steps are absent. In the convenience area, genes related to different stages of fruit softening are identified. Conclusion: This large EST resource will allow researchers to undertake the tremendous challenge of understanding the molecular basis of genetic diversity in the Actinidia genus as well as provide an EST resource for comparative fruit genomics. The various bioinformatics analyses we have undertaken demonstrates the extent of coverage of ESTs for genes encoding different biochemical pathways in Actinidia. en
dc.language EN en
dc.publisher BioMed Central Ltd. en
dc.relation.ispartofseries BMC Genomics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.subject KIWI-FRUIT en
dc.subject CELL-WALL en
dc.subject STORAGE PROTEINS en
dc.title Analysis of expressed sequence tags from Actinidia: Applications of a cross species EST database for gene discovery in the areas of flavor, health, color and ripening en
dc.type Journal Article en
dc.identifier.doi 10.1186/1471-2164-9-351 en
pubs.begin-page 1 en
pubs.volume 9 en
dc.rights.holder Copyright: BioMed Central Ltd. en
dc.identifier.pmid 18655731 en
pubs.end-page 26 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 215952 en Science en Biological Sciences en
pubs.number 351 en
pubs.record-created-at-source-date 2012-02-14 en
pubs.dimensions-id 18655731 en

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