dc.contributor.author |
Grupcheva, CN |
en |
dc.contributor.author |
Laux, WT |
en |
dc.contributor.author |
Rupenthal, Ilva |
en |
dc.contributor.author |
McGhee, Jennifer |
en |
dc.contributor.author |
McGhee, Charles |
en |
dc.contributor.author |
Green, Colin |
en |
dc.coverage.spatial |
United States |
en |
dc.date.accessioned |
2012-03-04T22:00:27Z |
en |
dc.date.issued |
2012-03 |
en |
dc.identifier.citation |
Investigative Ophthalmology & Visual Science 53(3):1130-1138 Mar 2012 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/12740 |
en |
dc.description.abstract |
Purpose. Gap junctions play a major role in corneal wound healing. This study used reproducible models of corneal wound healing to evaluate the effect of a gap junction channel modulator, connexin43 (Cx43) antisense oligodeoxynucleotides (AsODN), on corneal healing dynamics. Methods. A mechanical scrape wound model was used to evaluate Cx43 AsODN penetration and initial wound reepithelialization 12 hours postsurgery. Thereafter, detailed analyses of corneal edema, inflammation, and healing were performed in an excimer laser surface ablation model. In vivo confocal microscopy determined clinical parameters (edema, haze) and cellular changes (stromal hypercellularity, reepithelialization), whereas histology and immunohistochemistry were used to quantify stromal edema, inflammation, and reepithelialization. Results. Cx43 AsODN penetrated through the hydrophilic stroma where the epithelium had been removed and accumulated in the basal epithelium close to the wound edge. Twelve hours after scrape wounding, Cx43 AsODN–treated eyes showed a significant reduction in wound area compared with the vehicle alone (1.59 ± 0.37 and 2.29 ± 0.58 mm2, respectively, P < 0.01). After excimer laser ablation, stromal edema and inflammation were reduced, with endothelial structures being clearly visible, and reepithelialization rates were again increased in Cx43 AsODN–treated eyes. Histologic analysis confirmed reduced edema in the central wound site and at the periphery of treated corneas (P < 0.05), whereas immunohistochemistry showed lower Cx43 levels (P < 0.05), reduced myofibroblast activation, and improved epithelial basal lamina deposition in antisense-treated wounds (P < 0.01). Conclusions. Application of Cx43 AsODN to the cornea reduces stromal edema and inflammation, promoting faster wound closure and a more uniform repair of the epithelial basal lamina after laser ablation. |
en |
dc.language |
eng |
en |
dc.relation.ispartofseries |
Investigative Ophthalmology & Visual Science |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.journalofvision.org/site/misc/forauthors.xhtml http://www.sherpa.ac.uk/romeo/issn/0146-0404/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Animals |
en |
dc.subject |
Connexin 43 |
en |
dc.subject |
Cornea |
en |
dc.subject |
Disease Models, Animal |
en |
dc.subject |
Eye Injuries |
en |
dc.subject |
Female |
en |
dc.subject |
Gap Junctions |
en |
dc.subject |
Immunohistochemistry |
en |
dc.subject |
Microscopy, Confocal |
en |
dc.subject |
Oligodeoxyribonucleotides |
en |
dc.subject |
Rats |
en |
dc.subject |
Rats, Wistar |
en |
dc.subject |
Wound Healing |
en |
dc.title |
Improved corneal wound healing through modulation of gap junction communication using connexin43-specific antisense oligodeoxynucleotides. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1167/iovs.11-8711 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
1130 |
en |
pubs.volume |
53 |
en |
dc.identifier.pmid |
22247467 |
en |
pubs.end-page |
1138 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
277089 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
School of Medicine |
en |
pubs.org-id |
Ophthalmology Department |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
dc.identifier.eissn |
1552-5783 |
en |
dc.identifier.pii |
iovs.11-8711 |
en |
pubs.record-created-at-source-date |
2012-10-15 |
en |
pubs.dimensions-id |
22247467 |
en |