dc.contributor.advisor |
Cooper, Garth |
en |
dc.contributor.advisor |
Bass, John |
en |
dc.contributor.author |
Clarke, Andrew John |
en |
dc.date.accessioned |
2007-08-06T06:35:22Z |
en |
dc.date.available |
2007-08-06T06:35:22Z |
en |
dc.date.issued |
2004 |
en |
dc.identifier |
THESIS 05-026 |
en |
dc.identifier.citation |
Thesis (PhD--Biological Sciences)--University of Auckland, 2004 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/1274 |
en |
dc.description |
Full text is available to authenticated members of The University of Auckland only. |
en |
dc.description.abstract |
This study aimed to identify genes or gene products associated with high lean muscle mass in bovines that may serve as potential markers for selection. An animal with a genetic predisposition to high lean muscle mass, the Belgium Blue, was chosen as a model to compare with the Holstein Friesian, a model that does not. This study aimed to identify myofibrillar proteins whose expression, or relative expression, is indicative of the potential musculature of an animal. Such a protein may not be directly related to the action of the gdf-8 gene but may be an allele that segregated with the mutant gdf-8 during selective breeding aimed at establishing breeds of extreme muscled cattle. 2D-PAGE analysis was utilized to compare the exhibited skeletal muscle proteome between the two animal types at two stages of foetal development. A previously uncharacterised polymorphism of a high expression myofibrillar protein, myosin light chain 1 fast (MLC-1f), was observed.
The characterisation of this polymorphism revealed a two-amino acid insertion in a part of the protein that has been implicated in modulating myosin ATPase activity. Furthermore, this polymorphism was shown to be the product of two alleles that are co-dominant. Screening studies were carried out on selected herds and showed a very high frequency of one allele.
Both isoforms of MLC-1f were produced by recombinant means and purified. The recombinant proteins were exchanged into purified myosin hexamers that were then subject to assays measuring ATP consumption. The sensitivity of the assay utilized could not reveal any significant difference in ATPase activity between hexamers containing one or the other isoform. |
en |
dc.language.iso |
en |
en |
dc.publisher |
ResearchSpace@Auckland |
en |
dc.relation.ispartof |
PhD Thesis - University of Auckland |
en |
dc.relation.isreferencedby |
UoA99145593714002091 |
en |
dc.rights |
Restricted Item. Available to authenticated members of The University of Auckland. |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Identification and characterisation of a bovine myosin light chain-1 fast polymorphism |
en |
dc.type |
Thesis |
en |
thesis.degree.discipline |
Biological Sciences |
en |
thesis.degree.grantor |
The University of Auckland |
en |
thesis.degree.level |
Doctoral |
en |
thesis.degree.name |
PhD |
en |
dc.rights.holder |
Copyright: The author |
en |
dc.identifier.wikidata |
Q112859546 |
|