dc.contributor.author |
Askarian Amiri, Effat |
en |
dc.contributor.author |
Pel, HJ |
en |
dc.contributor.author |
Guévremont, D |
en |
dc.contributor.author |
McCaughan, KK |
en |
dc.contributor.author |
Poole, ES |
en |
dc.contributor.author |
Sumpter, VG |
en |
dc.contributor.author |
Tate, WP |
en |
dc.date.accessioned |
2012-03-08T19:01:44Z |
en |
dc.date.issued |
2000 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/13458 |
en |
dc.description.abstract |
The yeast Saccharomyces cerevisiae mitochondrial release factor was expressed from the cloned MRF1 gene, purified from inclusion bodies, and refolded to give functional activity. The gene encoded a factor with release activity that recognized cognate stop codons in a termination assay with mitochondrial ribosomes and in an assay with Escherichia coli ribosomes. The noncognate stop codon, UGA, encoding tryptophan in mitochondria, was recognized weakly in the heterologous assay. The mitochondrial release factor 1 protein bound to bacterial ribosomes and formed a cross-link with the stop codon within a mRNA bound in a termination complex. The affinity was strongly dependent on the identity of stop signal. Two alleles of MRF1 that contained point mutations in a release factor 1 specific region of the primary structure and that in vivo compensated for mutations in the decoding site rRNA of mitochondrial ribosomes were cloned, and the expressed proteins were purified and refolded. The variant proteins showed impaired binding to the ribosome compared with mitochondrial release factor 1. This structural region in release factors is likely to be involved in codon-dependent specific ribosomal interactions. |
en |
dc.language |
English |
en |
dc.relation.ispartofseries |
Journal of Biological Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Functional characterization of yeast mitochondrial release factor 1. |
en |
dc.type |
Journal Article |
en |
pubs.issue |
23 |
en |
pubs.begin-page |
17241 |
en |
pubs.volume |
275 |
en |
pubs.end-page |
17248 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
304988 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Auckland Cancer Research |
en |
pubs.record-created-at-source-date |
2012-02-27 |
en |