On How Degeneration Influences Load-Bearing In The Cartilage-Bone System: A Microstructural And Micro-Mechanical Study.

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dc.contributor.author Thambyah, Ashvin en
dc.contributor.author Broom, Neil en
dc.date.accessioned 2012-03-08T22:56:57Z en
dc.date.issued 2007 en
dc.identifier.citation Osteoarthritis Cartilage 15(12):1410-1423 Dec 2007 en
dc.identifier.issn 1063-4584 en
dc.identifier.uri http://hdl.handle.net/2292/13571 en
dc.description.abstract Objective: This study investigated the microanatomical response to compression of intact and degenerate cartilage-on-bone samples with the aim of elucidating the functional consequences of articular surface disruption and related matrix changes. Method: Two groups of mature bovine patellae were identified at the time of harvest; those with intact cartilage and those with cartilage exhibiting mild to severe degeneration. Cartilage-on-bone samples were statically compressed (7 MPa) to near-equilibrium using an 8-mm diameter cylindrical indenter, and then formalin-fixed in this deformed state. Following mild decalcification full-depth cartilage–bone sections, incorporating the indentation profile and beyond, were studied in their fully hydrated state using differential interference contrast optical microscopy (DIC). Results: Differences in matrix texture, degree of disruption of the articular surface layer (or its complete absence), number of tidemarks and absence or presence of vascularization of the calcified cartilage zone were all observable features that provided clear differentiation between the normal and degenerate tissues. Under load a chevron-type shear discontinuity characterized those samples in which the strain-limiting surface layer was still largely intact. The extent to which this shear discontinuity advanced into the adjacent non-directly loaded cartilage continuum was influenced by the integrity of the cartilage general matrix. For those tissues deficient in a strain-limiting articular surface there was no shear discontinuity, the cartilage deformation field was instead shaped primarily by its osteochondral attachment and a laterally-directed compressive collapse of a much weakened matrix. In the degenerate samples the altered matrix textures associated with different regions of the deformation field are interpreted in terms of an intrinsic fibrillar architecture that is weakened by two fundamental processes: (1) a de-structuring resulting from a reduction in connectivity between fibrils and (2) subsequent aggregation of these now disconnected fibrils. Conclusion: DIC microscopy provides a high-resolution description of the integrated osteochondral tissue system across the full continuum of matrices, from normal to severely degenerate. Our study demonstrates the important functional role played by the strain-limiting articular surface, the consequences associated with its disruption, as well as the loss of effective stress transmission associated with a ‘de-structured’ general matrix. The study also provides new insights into the integration of cartilage with both its subchondral substrate and the wider continuum of non-directly loaded cartilage. en
dc.publisher Elsevier en
dc.relation.ispartofseries Osteoarthritis and Cartilage en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/1063-4584/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title On How Degeneration Influences Load-Bearing In The Cartilage-Bone System: A Microstructural And Micro-Mechanical Study. en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.joca.2007.05.006 en
pubs.issue 12 en
pubs.begin-page 1410 en
pubs.volume 15 en
dc.rights.holder Copyright: Elsevier en
dc.identifier.pmid 17689989 en
pubs.author-url http://www.sciencedirect.com/science/article/pii/S1063458407001719 en
pubs.end-page 1423 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 73781 en
pubs.org-id Engineering en
pubs.org-id Chemical and Materials Eng en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 17689989 en

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