Amplification of DNA from preserved specimens shows blowflies were preadapted for the rapid evolution of insecticide resistance

Show simple item record Hartley, CJ en Newcomb, Richard en Russell, RJ en Yong, CG en Stevens, JR en Yeates, DK en La Salle, J en Oakeshott, JG en 2012-03-08T23:09:54Z en 2006 en
dc.identifier.citation Proceedings of the National Academy of Sciences of USA 103(23):8757-8762 2006 en
dc.identifier.citation Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.identifier.issn 0027-8424 en
dc.identifier.uri en
dc.description.abstract Mutations of esterase 3 confer two forms of organophosphate resistance on contemporary Australasian Lucilia cuprina. One form, called diazinon resistance, is slightly more effective against commonly used insecticides and is now more prevalent than the other form, called malathion resistance. We report here that the single amino acid replacement associated with diazinon resistance and two replacements associated with malathion resistance also occur in esterase 3 in the sibling species Lucilia sericata, suggesting convergent evolution around a finite set of resistance options. We also find parallels between the species in the geographic distributions of the polymorphisms: In both cases, the diazinon-resistance change is absent or rare outside Australasia where insecticide pressure is lower, whereas the changes associated with malathion resistance are widespread. Furthermore, PCR analysis of pinned specimens of Australasian L. cuprina collected before the release of organophosphate insecticides reveals no cases of the diazinon-resistance change but several cases of those associated with malathion resistance. Thus, the early outbreak of resistance in this species can be explained by the preexistence of mutant alleles encoding malathion resistance. The pinned specimen analysis also shows much higher genetic diversity at the locus before organophosphate use, suggesting that the subsequent sweep of diazinon resistance in Australasia has compromised the scope for the locus to respond further to the ongoing challenge of the insecticides. en
dc.language EN en
dc.publisher The National Academy of Sciences of the USA en
dc.relation.ispartofseries Proceedings of the National Academy of Sciences of the United States of America en
dc.rights.uri en
dc.subject Lucilia cuprina en
dc.subject Lucilia sericata en
dc.subject mutation en
dc.subject convergent evolution en
dc.subject organophosphates en
dc.subject GENETICS en
dc.subject CALLIPHORIDAE en
dc.subject POPULATIONS en
dc.subject HYDROLYSIS en
dc.subject MUTATIONS en
dc.subject SEQUENCES en
dc.title Amplification of DNA from preserved specimens shows blowflies were preadapted for the rapid evolution of insecticide resistance en
dc.type Journal Article en
dc.identifier.doi 10.1073/pnas.0509590103 en
pubs.issue 23 en
pubs.begin-page 8757 en
pubs.volume 103 en
dc.rights.holder Copyright: The National Academy of Sciences of the USA en
dc.identifier.pmid 16723400 en
pubs.end-page 8762 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 234061 en
pubs.record-created-at-source-date 2012-02-17 en
pubs.dimensions-id 16723400 en

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