Extreme polyploidy in a large bacterium

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dc.contributor.author Mendell, JE en
dc.contributor.author Clements, Kendall en
dc.contributor.author Choat, John en
dc.contributor.author Angert, ER en
dc.date.accessioned 2012-03-09T01:38:22Z en
dc.date.issued 2008 en
dc.identifier.citation P NATL ACAD SCI USA 105(18):6730-6734 06 May 2008 en
dc.identifier.issn 1091-6490 en
dc.identifier.uri http://hdl.handle.net/2292/13604 en
dc.description.abstract Cells rely on diffusion to move metabolites and biomolecules. Diffusion is highly efficient but only over short distances. Although eukaryotic cells have broken free of diffusion-dictated constraints on cell size, most bacteria and archaea are forced to remain small. Exceptions to this rule are found among the bacterial symbionts of surgeonfish; Epulopiscium spp. are cigar-shaped cells that reach lengths in excess of 600 m. A large Epulopiscium contains thousands of times more DNA than a bacterium such as Escherichia coli, but the composition of this DNA is not well understood. Here, we present evidence that Epulopiscium contains tens of thousands of copies of its genome. Using quantitative, single-cell PCR assays targeting single-copy genes, we have determined that copy number is positively correlated with Epulopiscium cell size. Although other bacteria are known to possess multiple genomes, polyploidy of the magnitude observed in Epulopiscium is unprecedented. The arrangement of genomes around the cell periphery may permit regional responses to local stimuli, thus allowing Epulopiscium to maintain its unusually large size. Surveys of the sequences of single-copy genes (dnaA, recA, and ftsZ) revealed genetic homogeneity within a cell consistent with only a small amount ( 1%) of the parental DNA being transferred to the next generation. The results also suggest that the abundance of genome copies in Epulopiscium may allow for an unstable genetic feature, a long mononucleotide tract, in an essential gene. With the evolution of extreme polyploidy and large cell size, Epulopiscium has acquired some of the advantages of eukaryotic cells. en
dc.publisher PNAS en
dc.relation.ispartofseries Proceedings of the National Academy of Sciences of the United States of America en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1091-6490/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Extreme polyploidy in a large bacterium en
dc.type Journal Article en
dc.identifier.doi 10.1073/pnas.0707522105 en
pubs.issue 18 en
pubs.begin-page 6730 en
pubs.volume 105 en
dc.rights.holder Copyright: PNAS en
dc.identifier.pmid 18445653 en
pubs.end-page 6734 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 95911 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 18445653 en

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