dc.contributor.author |
Lilly, ST |
en |
dc.contributor.author |
Drummond, Revel |
en |
dc.contributor.author |
Pearson, Michael |
en |
dc.contributor.author |
MacDiarmid, Robin |
en |
dc.date.accessioned |
2012-03-12T20:07:33Z |
en |
dc.date.issued |
2011-03 |
en |
dc.identifier.citation |
MOL PLANT MICROBE IN 24(3):294-304 Mar 2011 |
en |
dc.identifier.issn |
0894-0282 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/13972 |
en |
dc.description.abstract |
Real-time quantitative polymerase chain reaction (qPCR) of complementary DNA is now a standard method for studies of gene expression. However, qPCR can identify genuine variation only when transcript quantities are accurately normalized to an appropriate reference. To identify the most reliable reference genes for transcript quantification by qPCR, we describe a systematic evaluation of candidate reference genes of Arabidopsis thaliana ecotype Columbia-0 (Col-0). Twelve genes were selected for transcript stability studies by qPCR of complementary DNA prepared from Arabidopsis leaf tissue infected with one of five plant viruses (Cauliflower mosaic virus, Tobacco mosaic virus, Tomato spotted wilt virus, Turnip mosaic virus, and Turnip yellow mosaic virus). The F-box family protein, elongation factor 1-α, sand family protein, and protodermal factor 2 gene transcripts showed the most stable accumulation, whereas a traditionally used reference gene, Actin8, showed the least stable accumulation as measured by the geNorm algorithm. The data furnish plant virologists with reference genes for normalization of qPCR-derived gene expression in virus-infected Arabidopsis and will be beneficial to the selection and design of primers targeting orthologous genes in other plant species. |
en |
dc.language |
EN |
en |
dc.publisher |
The American Phytopathological Society |
en |
dc.relation.ispartofseries |
Molecular Plant-Microbe Interactions |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0894-0282/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
TIME RT-PCR |
en |
dc.subject |
INTERNAL CONTROL GENES |
en |
dc.subject |
SYSTEMATIC VALIDATION |
en |
dc.subject |
HOUSEKEEPING GENES |
en |
dc.subject |
EXPRESSION |
en |
dc.subject |
PLANTS |
en |
dc.subject |
VARIABILITY |
en |
dc.subject |
SELECTION |
en |
dc.subject |
PROTEINS |
en |
dc.subject |
POTATO |
en |
dc.title |
Identification and Validation of Reference Genes for Normalization of Transcripts from Virus-Infected Arabidopsis thaliana |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1094/MPMI-10-10-0236 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
294 |
en |
pubs.volume |
24 |
en |
dc.rights.holder |
Copyright: The American Phytopathological Society |
en |
dc.identifier.pmid |
21091160 |
en |
pubs.end-page |
304 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
206879 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.record-created-at-source-date |
2012-02-20 |
en |
pubs.dimensions-id |
21091160 |
en |