ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light

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dc.contributor.author Kim, Woong en
dc.contributor.author Fujiwara, S en
dc.contributor.author Suh, S en
dc.contributor.author Kim, J en
dc.contributor.author Kim, Y en
dc.contributor.author Han, L en
dc.contributor.author David, Karine en
dc.contributor.author Putterill, Joanna en
dc.contributor.author Nam, H en
dc.contributor.author Somers, D en
dc.date.accessioned 2012-03-12T23:24:55Z en
dc.date.issued 2007 en
dc.identifier.citation NATURE 449(7160):356-+ 20 Sep 2007 en
dc.identifier.issn 0028-0836 en
dc.identifier.uri http://hdl.handle.net/2292/14078 en
dc.description.abstract The circadian clock is essential for coordinating the proper phasing of many important cellular processes. Robust cycling of key clock elements is required to maintain strong circadian oscillations of these clock-controlled outputs. Rhythmic expression of the Arabidopsis thaliana F-box protein ZEITLUPE (ZTL) is necessary to sustain a normal circadian period by controlling the proteasome-dependent degradation of a central clock protein, TIMING OF CAB EXPRESSION 1 (TOC1)1, 2. ZTL messenger RNA is constitutively expressed, but ZTL protein levels oscillate with a threefold change in amplitude through an unknown mechanism3. Here we show that GIGANTEA (GI) is essential to establish and sustain oscillations of ZTL by a direct protein–protein interaction. GI, a large plant-specific protein with a previously undefined molecular role, stabilizes ZTL in vivo. Furthermore, the ZTL–GI interaction is strongly and specifically enhanced by blue light, through the amino-terminal flavin-binding LIGHT, OXYGEN OR VOLTAGE (LOV) domain of ZTL. Mutations within this domain greatly diminish ZTL–GI interactions, leading to strongly reduced ZTL levels. Notably, a C82A mutation in the LOV domain, implicated in the flavin-dependent photochemistry, eliminates blue-light-enhanced binding of GI to ZTL. These data establish ZTL as a blue-light photoreceptor, which facilitates its own stability through a blue-light-enhanced GI interaction. Because the regulation of GI transcription is clock-controlled, consequent GI protein cycling confers a post-translational rhythm on ZTL protein. This mechanism of establishing and sustaining robust oscillations of ZTL results in the high-amplitude TOC1 rhythms necessary for proper clock function. en
dc.publisher Nature Publishing Group en
dc.relation.ispartofseries Nature en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0028-0836/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title ZEITLUPE is a circadian photoreceptor stabilized by GIGANTEA in blue light en
dc.type Journal Article en
dc.identifier.doi 10.1038/nature06132 en
pubs.begin-page 356 en
pubs.volume 449 en
dc.rights.holder Copyright: Nature Publishing Group en
dc.identifier.pmid 17704763 en
pubs.end-page 360 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 77087 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 17704763 en

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