Enhancing absorption of fluorescein and LHRH across hindgut epithelia in a marsupial, the common brushtail possum Trichosurus vulpecula

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dc.contributor.author Wen, Jingyuan en
dc.contributor.author McLeod, BJ en
dc.contributor.author Tucker, IG en
dc.contributor.author Davies, N en
dc.contributor.author Ledger, R en
dc.contributor.author Butt, AG en
dc.date.accessioned 2012-03-15T00:38:28Z en
dc.date.issued 2007 en
dc.identifier.citation Journal of Pharmaceutical Sciences 96(9):2262-2273 2007 en
dc.identifier.issn 0022-3549 en
dc.identifier.uri http://hdl.handle.net/2292/14416 en
dc.description.abstract We have identified differences in transport properties of intestinal epithelia in the marsupial brushtail possum, compared to eutherian mammals. To determine whether differences in its permeability to hydrophilic compounds also occur, the absorption of sodium fluorescein and luteinizing hormone releasing hormone (LHRH) was assessed in vitro and the ability of chemical enhancers and a metabolic inhibitor to promote their absorption investigated. The apparent permeability of colonic and caecal tissues to fluorescein and LHRH and transepithelial resistance (Rt) in the absence or presence of ethylenediamine tetra-acetic acid (EDTA), sodium deoxycholic acid (SDA), dithiothreitol (DTT), polyacrylic acids (PAA), or the inhibitor bacitracin were determined. The effects of SDA and/or DTT on adherent mucus and the release of lactate dehydrogenase (LDH) were also assessed. In the absence of treatment, both tissues had comparable amounts of adherent mucus, Rt and low permeabilities to fluorescein and LHRH. All chemical enhancers increased fluorescein permeability, but SDA at concentrations >0.5 mM also induced LDH release. DTT alone and in combination with SDA reduced the amount of adherent mucus. Bacitracin inhibited LHRH metabolism and increased LHRH permeability. These data indicate that the possum hindgut epithelium represents a significant barrier to the uptake of hydrophilic compounds, similar to that in eutherians. en
dc.description.uri http://www3.interscience.wiley.com/cgi-bin/abstract/114264178/ABSTRACT?CRETRY=1&SRETRY=0 en
dc.publisher Wiley-Blackwell en
dc.relation.ispartofseries Journal of Pharmaceutical Sciences en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0022-3549/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Enhancing absorption of fluorescein and LHRH across hindgut epithelia in a marsupial, the common brushtail possum Trichosurus vulpecula en
dc.type Journal Article en
dc.identifier.doi 10.1002/jps.20882 en
pubs.issue 9 en
pubs.begin-page 2262 en
pubs.volume 96 en
dc.rights.holder Copyright: Wiley-Blackwell en
dc.identifier.pmid 17506515 en
pubs.end-page 2273 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 75529 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Pharmacy en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 17506515 en

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