Noninvasive biophotonic imaging for studies of infectious disease.

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dc.contributor.author Andreu, N en
dc.contributor.author Zelmer, A en
dc.contributor.author Wiles, Siouxsie en
dc.coverage.spatial England en
dc.date.accessioned 2012-03-15T19:56:11Z en
dc.date.issued 2011-03 en
dc.identifier.citation FEMS Microbiology Reviews 35(2):360-394 Mar 2011 en
dc.identifier.issn 0168-6445 en
dc.identifier.uri http://hdl.handle.net/2292/14454 en
dc.description.abstract According to World Health Organization estimates, infectious organisms are responsible for approximately one in four deaths worldwide. Animal models play an essential role in the development of vaccines and therapeutic agents but large numbers of animals are required to obtain quantitative microbiological data by tissue sampling. Biophotonic imaging (BPI) is a highly sensitive, nontoxic technique based on the detection of visible light, produced by luciferase-catalysed reactions (bioluminescence) or by excitation of fluorescent molecules, using sensitive photon detectors. The development of bioluminescent/fluorescent microorganisms therefore allows the real-time noninvasive detection of microorganisms within intact living animals. Multiple imaging of the same animal throughout an experiment allows disease progression to be followed with extreme accuracy, reducing the number of animals required to yield statistically meaningful data. In the study of infectious disease, the use of BPI is becoming widespread due to the novel insights it can provide into established models, as well as the impact of the technique on two of the guiding principles of using animals in research, namely reduction and refinement. Here, we review the technology of BPI, from the instrumentation through to the generation of a photonic signal, and illustrate how the technique is shedding light on infection dynamics in vivo. en
dc.language eng en
dc.publisher Blackwell en
dc.relation.ispartofseries FEMS Microbiology Reviews en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0168-6445/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Animals en
dc.subject Bacteria en
dc.subject Communicable Diseases en
dc.subject Diagnostic Imaging en
dc.subject Fungi en
dc.subject Humans en
dc.subject Luciferases en
dc.subject Luminescent Measurements en
dc.subject Mice en
dc.subject Parasites en
dc.subject Viruses en
dc.title Noninvasive biophotonic imaging for studies of infectious disease. en
dc.type Journal Article en
dc.identifier.doi 10.1111/j.1574-6976.2010.00252.x en
pubs.issue 2 en
pubs.begin-page 360 en
pubs.volume 35 en
dc.rights.holder Copyright: Blackwell en
dc.identifier.pmid 20955395 en
pubs.end-page 394 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Review en
pubs.elements-id 165639 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id Science en
pubs.org-id Science Research en
pubs.org-id Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1574-6976 en
pubs.record-created-at-source-date 2012-02-14 en
pubs.dimensions-id 20955395 en


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