dc.contributor.author |
Russell, DM |
en |
dc.contributor.author |
Garry, EM |
en |
dc.contributor.author |
Taberner, Andrew |
en |
dc.contributor.author |
Barrett, Carolyn |
en |
dc.contributor.author |
Paton, Julian |
en |
dc.contributor.author |
Budgett, David |
en |
dc.contributor.author |
Malpas, Simon |
en |
dc.date.accessioned |
2012-03-15T22:36:39Z |
en |
dc.date.issued |
2012 |
en |
dc.identifier.citation |
Journal of Neuroscience Methods 204(2):242-248 2012 |
en |
dc.identifier.issn |
0165-0270 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/14486 |
en |
dc.description.abstract |
The ability to monitor tissue oxygen concentration in a specific region of the brain in a freely moving animal could provide a new paradigm in neuroscience research. We have developed a fully implantable telemetry system for the continuous and chronic recording of brain tissue oxygen (PO2,BR) in conscious animals. A telemetry system with a sampling rate of 2 kHz was combined with a miniaturized potentiostat to amperiometrically detect oxygen concentration with carbon paste electrodes. Wireless power was employed to recharge the telemeter battery transcutaneously for potential lifetime monitoring. Rats were implanted with the telemeter in the peritoneal cavity and electrodes stereotaxically implanted into the brain (striatum or medulla oblongata). While the animals were living in their home cages the sensitivity to changes in oxygen was validated by repeatedly altering the inspired oxygen (10%, 100%, respectively) or a pharmacological stimulus (carbonic anhydrase inhibitor: acetazolamide 50 mg/kg IP). Basal level of PO2,BR was monitored for 3 weeks and showed good overall stability and good correlation to movement such as grooming. During hypoxia, PO2,BR decreased significantly by –51% ± 2% from baseline, whereas it increased by 34% ± 3% during hyperoxia. Following the systemic administration of acetazolamide, PO2,BR increased by 38% ± 4%. We propose this new technology provides a robust method to measure changes in oxygen concentration in specific areas of the brain, in conscious freely moving rats. The ability to track long term changes with disease progression or drug treatment may be enabled. |
en |
dc.publisher |
Elsevier |
en |
dc.relation.ispartofseries |
Journal of Neuroscience Methods |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0165-0270/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Carbon paste electrodes |
en |
dc.subject |
Brain tissue oxygen |
en |
dc.subject |
In vivo electrochemistry |
en |
dc.subject |
Telemetry |
en |
dc.subject |
Electrochemical Sensors |
en |
dc.title |
A fully implantable telemetry system for the chronic monitoring of brain tissue oxygen in freely moving rats |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.jneumeth.2011.11.019 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
242 |
en |
pubs.volume |
204 |
en |
dc.rights.holder |
Copyright: Elsevier |
en |
dc.identifier.pmid |
22123353 |
en |
pubs.author-url |
http://www.sciencedirect.com/science/article/pii/S0165027011006960 |
en |
pubs.end-page |
248 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
245582 |
en |
pubs.org-id |
Bioengineering Institute |
en |
pubs.org-id |
ABI Associates |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Physiology Division |
en |
dc.identifier.eissn |
1872-678X |
en |
pubs.record-created-at-source-date |
2011-11-27 |
en |
pubs.dimensions-id |
22123353 |
en |