Illuminating the molecular basis of diabetic arteriopathy: A proteomic comparison of aortic tissue from diabetic and healthy rats

Show simple item record Jüllig, M en Chen, X en Middleditch, Martin en Vazhoor, G en Hickey, Anthony en Gong, D en Lu, Jun en Zhang, Shaoping en Phillips, Anthony en Cooper, Garth en 2012-03-16T02:24:15Z en 2010 en
dc.identifier.citation Proteomics 10(18):3367-3378 2010 en
dc.identifier.issn 1615-9853 en
dc.identifier.uri en
dc.description.abstract Arterial disease is a major diabetic complication, yet the component molecular mechanisms of diabetic arteriopathy remain poorly understood. In order to identify major proteins/pathways implicated in diabetic arteriopathy, we studied the effect of 16-wk untreated streptozotocin-induced diabetes on the rat aortic proteome. Specific protein levels in isolated aortas were compared in six discrete, pair-wise (streptozotocin-diabetic and non-diabetic age-matched controls) experiments in which individual proteins were identified and quantified by iTRAQ combined with LC-MS/MS. A total of 398 unique non-redundant proteins were identified in at least one experiment and 208 were detected in three or more. Between-group comparisons revealed significant changes or trends towards changes in relative abundance of 51 proteins (25 increased, 26 decreased). Differences in levels of selected proteins were supported by Western blotting and/or enzyme assays. The most prominent diabetes-associated changes were in groups of proteins linked to oxidative stress responses and the structure/function of myofibrils and microfilaments. Indexes of mitochondrial content were measurably lower in aortic tissue from diabetic animals. Functional cluster analysis also showed decreased levels of glycolytic enzymes and mitochondrial electron transport system-complex components. These findings newly implicate several proteins/functional pathways in the pathogenesis of arteriosclerosis/diabetic arteriopathy. en
dc.publisher WILEY-VCH Verlag GmbH & Co. en
dc.relation.ispartofseries Proteomics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Illuminating the molecular basis of diabetic arteriopathy: A proteomic comparison of aortic tissue from diabetic and healthy rats en
dc.type Journal Article en
dc.identifier.doi 10.1002/pmic.201000276 en
pubs.issue 18 en
pubs.begin-page 3367 en
pubs.volume 10 en
dc.rights.holder Copyright: WILEY-VCH Verlag GmbH & Co. en
dc.identifier.pmid 20707005 en
pubs.end-page 3378 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 152898 en Science en Biological Sciences en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1615-9861 en
pubs.record-created-at-source-date 2012-03-16 en
pubs.dimensions-id 20707005 en

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