Abstract:
Cyclic AMP was extracted from P. radiate tissue by homogenization in 0.1M-HCl at 100C with added [8-3H] cyclic AMP. The nucleotide was purified by the successive use of anion exchange chromatography, gel filtration and chromatography on PEI cellulose in two solvent systems.
KD fro cyclic AMP in the system isobutanol/water was determined for a range of temperatures and the compound of similar chromatographic behavior to [8-3H] cyclic AMP was identified as cyclic AMP by countercurrent distributions. Mass was determined by the gilman assay.