Sex pheromone evolution is associated with differential regulation of the same desaturase gene in two genera of leafroller moths

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Show simple item record Albre, J en Liénard, MA en Sirey, TM en Schmidt, S en Tooman, LK en Carraher, C en Greenwood, David en Löfstedt, C en Newcomb, Richard en
dc.coverage.spatial United States en 2012-03-18T19:32:40Z en 2012-01 en
dc.identifier.citation PLoS Genet 8(1):e1002489 Jan 2012 en
dc.identifier.issn 1553-7390 en
dc.identifier.uri en
dc.description.abstract Chemical signals are prevalent in sexual communication systems. Mate recognition has been extensively studied within the Lepidoptera, where the production and recognition of species-specific sex pheromone signals are typically the defining character. While the specific blend of compounds that makes up the sex pheromones of many species has been characterized, the molecular mechanisms underpinning the evolution of pheromone-based mate recognition systems remain largely unknown. We have focused on two sets of sibling species within the leafroller moth genera Ctenopseustis and Planotortrix that have rapidly evolved the use of distinct sex pheromone blends. The compounds within these blends differ almost exclusively in the relative position of double bonds that are introduced by desaturase enzymes. Of the six desaturase orthologs isolated from all four species, functional analyses in yeast and gene expression in pheromone glands implicate three in pheromone biosynthesis, two Δ9-desaturases, and a Δ10-desaturase, while the remaining three desaturases include a Δ6-desaturase, a terminal desaturase, and a non-functional desaturase. Comparative quantitative real-time PCR reveals that the Δ10-desaturase is differentially expressed in the pheromone glands of the two sets of sibling species, consistent with differences in the pheromone blend in both species pairs. In the pheromone glands of species that utilize (Z)-8-tetradecenyl acetate as sex pheromone component (Ctenopseustis obliquana and Planotortrix octo), the expression levels of the Δ10-desaturase are significantly higher than in the pheromone glands of their respective sibling species (C. herana and P. excessana). Our results demonstrate that interspecific sex pheromone differences are associated with differential regulation of the same desaturase gene in two genera of moths. We suggest that differential gene regulation among members of a multigene family may be an important mechanism of molecular innovation in sex pheromone evolution and speciation. en
dc.language eng en
dc.publisher Public Library of Science en
dc.relation.ispartofseries PLOS Genetics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.rights.uri en
dc.subject Animals en
dc.subject Evolution, Molecular en
dc.subject Fatty Acid Desaturases en
dc.subject Fatty Acids en
dc.subject Gene Expression Regulation en
dc.subject Genetic Speciation en
dc.subject Lepidoptera en
dc.subject Marriage en
dc.subject Phylogeny en
dc.subject Sequence Homology, Amino Acid en
dc.subject Sex Attractants en
dc.subject Sexual Behavior, Animal en
dc.subject Species Specificity en
dc.title Sex pheromone evolution is associated with differential regulation of the same desaturase gene in two genera of leafroller moths en
dc.type Journal Article en
dc.identifier.doi 10.1371/journal.pgen.1002489 en
pubs.issue 1 en
pubs.begin-page e1002489 en
pubs.volume 8 en
dc.rights.holder Copyright: 2012 Albre et al. en
dc.identifier.pmid 22291612 en
pubs.end-page e1002489 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 286091 en
dc.identifier.eissn 1553-7404 en
dc.identifier.pii PGENETICS-D-11-01672 en
pubs.record-created-at-source-date 2012-08-01 en
pubs.dimensions-id 22291612 en

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