dc.contributor.author |
Jain, R |
en |
dc.contributor.author |
Wu, Zimei |
en |
dc.contributor.author |
Tucker, IG |
en |
dc.date.accessioned |
2012-03-18T20:38:33Z |
en |
dc.date.issued |
2009 |
en |
dc.identifier.citation |
Journal of Pharmaceutical and Biomedical Analysis 50(5):841-846 05 Dec 2009 |
en |
dc.identifier.issn |
0731-7085 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/14598 |
en |
dc.description.abstract |
The aim of this study was to develop a stability-indicating HPLC assay for the determination of penethamate (PNT), an ester prodrug of benzylpenicillin (BP), in aqueous solutions. The method was validated by subjecting PNT to forced decomposition under stress conditions of acid, alkali, water hydrolysis and oxidation. A quenching solutionwas developed to limit degradation to negligible levels before and during the analysis. Both PNT and BPwere simultaneously determined and separated in presence of degradation products on a C18 column using a mobile phase consisting of methanol–acetonitrile–acetate buffer. Different degradation products were formed in the stress conditions. The peak purity indexes of PNT and BP obtained by diode array detection were >0.999, confirming the absence of other co-eluting substances. The assay was linear for both analytes in the concentration range 1–100 gmL−1. The LOD and LOQ of PNT were 0.03 and 0.09 gmL−1 respectively. Degradation of PNT followed pseudo-first-order kinetics with t1/2 of 43.6 min at pH 2.01 and 4.2 min at pH 9.31. In addition, the absence of BP in the acidic solutions of PNT emphasises the futility of monitoring BP to assess the stability of PNT. In conclusion, the assay is rapid and stability-indicating with adequate precision and accuracy, and in conjunction with the quenching solution, can be used for stability studies of PNT with simultaneous quantitation of BP. The degradation studies provide useful information for formulation development of PNT. |
en |
dc.publisher |
Elsevier |
en |
dc.relation.ispartofseries |
Journal of Pharmaceutical and Biomedical Analysis |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0731-7085/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
A stability-indicating HPLC assay with diode array detection for the determination of a benzylpenicillin prodrug in aqueous solutions |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.jpba.2009.06.046 |
en |
pubs.issue |
5 |
en |
pubs.begin-page |
841 |
en |
pubs.volume |
50 |
en |
dc.rights.holder |
Copyright: Elsevier |
en |
dc.identifier.pmid |
19640667 |
en |
pubs.end-page |
846 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
97012 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Pharmacy |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
19640667 |
en |