Replication functions of the F plasmid of Escherichia coli

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dc.contributor.advisor Bergquist, P. en
dc.contributor.advisor Lane, D. en
dc.contributor.author Gardner, Richard C. en
dc.date.accessioned 2007-08-18T09:38:39Z en
dc.date.available 2007-08-18T09:38:39Z en
dc.date.issued 1979 en
dc.identifier THESIS 80-007 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1979 en
dc.identifier.uri http://hdl.handle.net/2292/1463 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract The work in this thesis concerns the replication of the F plasmid of Escherichia coli and is addressed to the question: how many plasmid genes are involved in replication and partition of F, and what are their function? The techniques principally employed were the cloning of restriction fragments containing parts of the F genome, and transposition mutagenesis of mini-F derivatives. The results obtained can be summarised as follows: (1) The F plasmid contains a second, previously undiscovered, replicon which is located within a 3 kilobase segment of EcoRI fragment f7. The f7 replicon was shown to take over replication of the intact F plasmid when the primary (f5) replication system was inhibited. Replication of f7 plasmids was found to be partly defective in E. coli, especially in rich media. (2) Several temperature-sensitive mutations of F were mapped within the essential replication region (F43.8-47.0kb) of -EcoRI fragment f5. The isolation of temperature-sensitive mini-plasmids should greatly facilitate study of the nature of these lesions. (3) A mini-F plasmid was obtained which constitutes the smallest F derivative so far reported. This plasmid is deleted for the two known origins of replication, oriV and oriS, which suggests the existence of a third origin of replication within the f5 fragment. (4) A locus required for stability of f5 mini-plasmids was mapped to the F46.35-49.25kb region. Two possible functions are suggested for this locus. (5) The part of f5 that is responsible for the incompatibility reaction shown between IncFI group plasmids was found to map in the F47.6-49.25kb region. Therefore this area constitutes a third incompatibility region of F, distinct from the incA and incB regions defined by Kahn et al. (1978). en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA9921862014002091 en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Replication functions of the F plasmid of Escherichia coli en
dc.type Thesis en
thesis.degree.discipline Cell Biology en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.rights.holder Copyright: The author en


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