Live imaging of Runx1 expression in the dorsal aorta tracks the emergence of blood progenitors from endothelial cells

Show simple item record Lam, EYN en Hall, Christopher en Crosier, Philip en Crosier, Kathryn en Flores, MV en 2012-03-25T20:25:18Z en 2010-08-12 en
dc.identifier.citation BLOOD 116(6):909-914 12 Aug 2010 en
dc.identifier.issn 0006-4971 en
dc.identifier.uri en
dc.description.abstract Blood cells of an adult vertebrate are continuously generated by hematopoietic stem cells (HSCs) that originate during embryonic life within the aorta-gonad-mesonephros region. There is now compelling in vivo evidence that HSCs are generated from aortic endothelial cells and that this process is critically regulated by the transcription factor Runx1. By time-lapse microscopy of Runx1-enhanced green fluorescent protein transgenic zebrafish embryos, we were able to capture a subset of cells within the ventral endothelium of the dorsal aorta, as they acquire hemogenic properties and directly emerge as presumptive HSCs. These nascent hematopoietic cells assume a rounded morphology, transiently occupy the subaortic space, and eventually enter the circulation via the caudal vein. Cell tracing showed that these cells subsequently populated the sites of definitive hematopoiesis (thymus and kidney), consistent with an HSC identity. HSC numbers depended on activity of the transcription factor Runx1, on blood flow, and on proper development of the dorsal aorta (features in common with mammals). This study captures the earliest events of the transition of endothelial cells to a hemogenic endothelium and demonstrates that embryonic hematopoietic progenitors directly differentiate from endothelial cells within a living organism. (Blood. 2010;116(6):909-914) en
dc.language English en
dc.publisher The American Society of Hematolog en
dc.relation.ispartofseries Blood en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.subject Science & Technology en
dc.subject Life Sciences & Biomedicine en
dc.subject Hematology en
dc.subject TRANSITION en
dc.subject PRECURSOR en
dc.subject ONTOGENY en
dc.subject GENES en
dc.title Live imaging of Runx1 expression in the dorsal aorta tracks the emergence of blood progenitors from endothelial cells en
dc.type Journal Article en
dc.identifier.doi 10.1182/blood-2010-01-264382 en
pubs.issue 6 en
pubs.begin-page 909 en
pubs.volume 116 en
dc.rights.holder Copyright: The American Society of Hematology en
dc.identifier.pmid 20453160 en en
pubs.end-page 914 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 120192 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
dc.identifier.eissn 1528-0020 en
pubs.record-created-at-source-date 2012-03-26 en
pubs.dimensions-id 20453160 en

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