Intestinal differentiation in zebrafish requires Cdx1b, a functional equivalent of mammalian Cdx2

Show simple item record Flores, Maria en Hall, Christopher en Davidson, Alan en Singh, Primal en Mahagaonkar, Alhad en Crosier, Kathryn en Crosier, Philip en 2012-03-25T20:29:59Z en 2008 en
dc.identifier.citation GASTROENTEROLOGY 135(5):1665-1675 01 Nov 2008 en
dc.identifier.issn 0016-5085 en
dc.identifier.uri en
dc.description.abstract Background & Aims: The ParaHox transcription factor Cdx2 is an essential determinant of intestinal phenotype in mammals throughout development, in- fluencing gut function, homeostasis, and epithelial barrier integrity. Cdx2 expression demarcates the zones of intestinal stem cell proliferation in the adult gut, with deregulated expression implicated in intestinal metaplasia and cancer. However, in vivo analysis of these prospective roles has been limited because inactivation of Cdx2 in mice leads to preimplantation embryonic lethality. We used the zebrafish, a valuable model for studying gut development, to generate a system to further understanding of the role of Cdx2 in normal intestinal function and in disease states. Methods: We isolated and characterized the zebrafish cdx1b ortholog and analyzed its function by antisense morpholino gene knockdown. Results: We showed that zebrafish Cdx1b replaces the role of Cdx2 in gut development. Evolutionary studies have indicated that the zebrafish cdx2 loci were lost following the genome-wide duplication event that occurred in teleosts. Zebrafish Cdx1b is expressed exclusively in the developing intestine during late embryogenesis and regulates intestinal cell proliferation and terminal differentiation. Conclusions: This work established an in vivo system to explore further the activity of Cdx2 in the gut and its impact on processes such as inflammation and cancer. en
dc.publisher AGA Institute en
dc.relation.ispartofseries Gastroenterology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from en
dc.rights.uri en
dc.title Intestinal differentiation in zebrafish requires Cdx1b, a functional equivalent of mammalian Cdx2 en
dc.type Journal Article en
dc.identifier.doi 10.1053/j.gastro.2008.07.024, en
pubs.issue 5 en
pubs.volume 135 en
dc.rights.holder Copyright: AGA Institute en
dc.identifier.pmid 18804112 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 84976 en Medical and Health Sciences en Medical Sciences en Molecular Medicine en Science en Science Research en Maurice Wilkins Centre (2010-2014) en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 18804112 en

Files in this item

There are no files associated with this item.

Find Full text

This item appears in the following Collection(s)

Show simple item record


Search ResearchSpace