Longterm quiescent cells in the aged human subventricular neurogenic system specifically express GFAP-delta

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dc.contributor.author van den Berge, SA en
dc.contributor.author Middeldorp, J en
dc.contributor.author Zhang, CE en
dc.contributor.author Curtis, Maurice en
dc.contributor.author Leonard, BW en
dc.contributor.author Mastroeni, D en
dc.contributor.author Voorn, P en
dc.contributor.author van de Berg, WDJ en
dc.contributor.author Huitinga, I en
dc.contributor.author Hol, EM en
dc.date.accessioned 2012-03-25T22:15:28Z en
dc.date.issued 2010-06-01 en
dc.identifier.citation AGING CELL 9(3):313-326 01 Jun 2010 en
dc.identifier.issn 1474-9718 en
dc.identifier.uri http://hdl.handle.net/2292/15248 en
dc.description.abstract P>A main neurogenic niche in the adult human brain is the subventricular zone (SVZ). Recent data suggest that the progenitors that are born in the human SVZ migrate via the rostral migratory stream (RMS) towards the olfactory bulb (OB), similar to what has been observed in other mammals. A subpopulation of astrocytes in the SVZ specifically expresses an assembly-compromised isoform of the intermediate filament protein glial fibrillary acidic protein (GFAP-delta). To further define the phenotype of these GFAP-delta expressing cells and to determine whether these cells are present throughout the human subventricular neurogenic system, we analysed SVZ, RMS and OB sections of 14 aged brain donors (ages 74-93). GFAP-delta was expressed in the SVZ along the ventricle, in the RMS and in the OB. The GFAP-delta cells in the SVZ co-expressed the neural stem cell (NSC) marker nestin and the cell proliferation markers proliferating cell nuclear antigen (PCNA) and Mcm2. Furthermore, BrdU retention was found in GFAP-delta positive cells in the SVZ. In the RMS, GFAP-delta was expressed in the glial net surrounding the neuroblasts. In the OB, GFAP-delta positive cells co-expressed PCNA. We also showed that GFAP-delta cells are present in neurosphere cultures that were derived from SVZ precursors, isolated postmortem from four brain donors (ages 63-91). Taken together, our findings show that GFAP-delta is expressed in an astrocytic subpopulation in the SVZ, the RMS and the OB. Importantly, we provide the first evidence that GFAP-delta is specifically expressed in longterm quiescent cells in the human SVZ, which are reminiscent of NSCs. en
dc.language English en
dc.publisher Blackwell Publishing Ltd.; Anatomical Society of Great Britain and Ireland en
dc.relation.ispartofseries Aging Cell en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained fromhttp://www.sherpa.ac.uk/romeo/issn/1474-9718/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Science & Technology en
dc.subject Life Sciences & Biomedicine en
dc.subject Cell Biology en
dc.subject Geriatrics & Gerontology en
dc.subject adult neural stem cells en
dc.subject astrocytes en
dc.subject subventricular zone en
dc.subject rostral migratory stream en
dc.subject olfactory bulb en
dc.subject aging brain en
dc.subject NEURAL STEM-CELLS en
dc.subject OLFACTORY-BULB en
dc.subject PROGENITOR CELLS en
dc.subject HUMAN FOREBRAIN en
dc.title Longterm quiescent cells in the aged human subventricular neurogenic system specifically express GFAP-delta en
dc.type Journal Article en
dc.identifier.doi 10.1111/j.1474-9726.2010.00556.x en
pubs.issue 3 en
pubs.begin-page 313 en
pubs.volume 9 en
dc.rights.holder Copyright: Blackwell Publishing Ltd.; Anatomical Society of Great Britain and Ireland en
dc.identifier.pmid 20121722 en
pubs.end-page 326 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 119552 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Anatomy and Medical Imaging en
pubs.record-created-at-source-date 2012-03-26 en
pubs.dimensions-id 20121722 en

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