dc.contributor.author |
van den Berge, SA |
en |
dc.contributor.author |
Middeldorp, J |
en |
dc.contributor.author |
Zhang, CE |
en |
dc.contributor.author |
Curtis, Maurice |
en |
dc.contributor.author |
Leonard, BW |
en |
dc.contributor.author |
Mastroeni, D |
en |
dc.contributor.author |
Voorn, P |
en |
dc.contributor.author |
van de Berg, WDJ |
en |
dc.contributor.author |
Huitinga, I |
en |
dc.contributor.author |
Hol, EM |
en |
dc.date.accessioned |
2012-03-25T22:15:28Z |
en |
dc.date.issued |
2010-06-01 |
en |
dc.identifier.citation |
AGING CELL 9(3):313-326 01 Jun 2010 |
en |
dc.identifier.issn |
1474-9718 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/15248 |
en |
dc.description.abstract |
P>A main neurogenic niche in the adult human brain is the subventricular zone (SVZ). Recent data suggest that the progenitors that are born in the human SVZ migrate via the rostral migratory stream (RMS) towards the olfactory bulb (OB), similar to what has been observed in other mammals. A subpopulation of astrocytes in the SVZ specifically expresses an assembly-compromised isoform of the intermediate filament protein glial fibrillary acidic protein (GFAP-delta). To further define the phenotype of these GFAP-delta expressing cells and to determine whether these cells are present throughout the human subventricular neurogenic system, we analysed SVZ, RMS and OB sections of 14 aged brain donors (ages 74-93). GFAP-delta was expressed in the SVZ along the ventricle, in the RMS and in the OB. The GFAP-delta cells in the SVZ co-expressed the neural stem cell (NSC) marker nestin and the cell proliferation markers proliferating cell nuclear antigen (PCNA) and Mcm2. Furthermore, BrdU retention was found in GFAP-delta positive cells in the SVZ. In the RMS, GFAP-delta was expressed in the glial net surrounding the neuroblasts. In the OB, GFAP-delta positive cells co-expressed PCNA. We also showed that GFAP-delta cells are present in neurosphere cultures that were derived from SVZ precursors, isolated postmortem from four brain donors (ages 63-91). Taken together, our findings show that GFAP-delta is expressed in an astrocytic subpopulation in the SVZ, the RMS and the OB. Importantly, we provide the first evidence that GFAP-delta is specifically expressed in longterm quiescent cells in the human SVZ, which are reminiscent of NSCs. |
en |
dc.language |
English |
en |
dc.publisher |
Blackwell Publishing Ltd.; Anatomical Society of Great Britain and Ireland |
en |
dc.relation.ispartofseries |
Aging Cell |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
Details obtained fromhttp://www.sherpa.ac.uk/romeo/issn/1474-9718/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Cell Biology |
en |
dc.subject |
Geriatrics & Gerontology |
en |
dc.subject |
adult neural stem cells |
en |
dc.subject |
astrocytes |
en |
dc.subject |
subventricular zone |
en |
dc.subject |
rostral migratory stream |
en |
dc.subject |
olfactory bulb |
en |
dc.subject |
aging brain |
en |
dc.subject |
ROSTRAL MIGRATORY STREAM |
en |
dc.subject |
FIBRILLARY ACIDIC PROTEIN |
en |
dc.subject |
NEURAL STEM-CELLS |
en |
dc.subject |
ADULT HUMAN HIPPOCAMPUS |
en |
dc.subject |
OLFACTORY-BULB |
en |
dc.subject |
PROGENITOR CELLS |
en |
dc.subject |
INTERMEDIATE-FILAMENTS |
en |
dc.subject |
NEURONAL PROGENITORS |
en |
dc.subject |
CELLULAR COMPOSITION |
en |
dc.subject |
HUMAN FOREBRAIN |
en |
dc.title |
Longterm quiescent cells in the aged human subventricular neurogenic system specifically express GFAP-delta |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1111/j.1474-9726.2010.00556.x |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
313 |
en |
pubs.volume |
9 |
en |
dc.rights.holder |
Copyright: Blackwell Publishing Ltd.; Anatomical Society of Great Britain and Ireland |
en |
dc.identifier.pmid |
20121722 |
en |
pubs.end-page |
326 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
119552 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Anatomy and Medical Imaging |
en |
pubs.record-created-at-source-date |
2012-03-26 |
en |
pubs.dimensions-id |
20121722 |
en |