dc.contributor.author |
Greenwood, D |
en |
dc.contributor.author |
Jagger, DJ |
en |
dc.contributor.author |
Huang, L |
en |
dc.contributor.author |
Hoya, N |
en |
dc.contributor.author |
Thorne, PR |
en |
dc.contributor.author |
Wildman, SS |
en |
dc.contributor.author |
King, BF |
en |
dc.contributor.author |
Pak, K |
en |
dc.contributor.author |
Ryan, AF |
en |
dc.contributor.author |
Housley, GD |
en |
dc.date.accessioned |
2012-03-26T20:03:23Z |
en |
dc.date.available |
2012-03-26T20:03:23Z |
en |
dc.date.issued |
2007 |
en |
dc.identifier.citation |
Development 134(7):1407-1417 01 Apr 2007 |
en |
dc.identifier.issn |
0950-1991 |
en |
dc.identifier.other |
1477-9129 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/15434 |
en |
dc.description.abstract |
Type I and type II spiral ganglion neurons (SGN) innervate the inner and outer hair cells of the cochlea, respectively. This neural system is established by reorganization of promiscuous innervation of the hair cells, immediately before hearing is established. The mechanism for this synaptic reorganization is unresolved but probably includes regulation of trophic support between the hair cells and the neurons. We provide evidence that P2X receptors (ATP-gated ion channels) contribute such a mechanism in the neonatal rat cochlea. Single-cell quantitative RT-PCR identified the differential expression of two P2X receptor subunits, splice variant P2X2-3 and P2X3, in a 1:2 transcript ratio. Downregulation of this P2X2-3/3 receptor coincided with maturation of the SGN innervation of the hair cells. When the P2X2-3 and P2X3 subunits were co-expressed in Xenopus oocytes, the resultant P2X receptor properties corresponded to the SGN phenotype. This included enhanced sensitivity to ATP and extended agonist action. In P4 spiral ganglion explants, activation of the P2X receptor signaling pathway by ATP S or , MeATP inhibited BDNF-induced neurite outgrowth and branching. These findings indicate that P2X receptor signaling provides a mechanism for inhibiting neurotrophin support of SGN neurites when synaptic reorganization is occurring in the cochlea. |
en |
dc.publisher |
Company of Biologists |
en |
dc.relation.ispartofseries |
Development |
en |
dc.relation.isreplacedby |
2292/23228 |
en |
dc.relation.isreplacedby |
http://hdl.handle.net/2292/23228 |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: http://www.sherpa.ac.uk/romeo/issn/0950-1991/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.source.uri |
http://dx.doi.org/10.1242/dev.002279 |
en |
dc.title |
P2X Receptor Signaling Inhibits BDNF-Mediated Spiral Ganglion Neuron Development in the Neonatal Rat Cochlea |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1242/dev.002279 |
en |
pubs.begin-page |
1407 |
en |
pubs.volume |
134 |
en |
dc.rights.holder |
Copyright: Company of Biologists |
en |
pubs.end-page |
1417 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.elements-id |
75574 |
en |
pubs.org-id |
Faculty of Medical & Hlth Sci |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Anatomy wth Radiology Division |
en |
pubs.org-id |
Physiology Division |
en |
pubs.org-id |
Population Health |
en |
pubs.org-id |
SOPH General Admin |
en |