A generic method to identify plant viruses by high-resolution tandem mass spectrometry of their coat proteins

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dc.contributor.author Blouin, Arnaud en
dc.contributor.author Greenwood, David en
dc.contributor.author Chavan, Ramesh en
dc.contributor.author Pearson, Michael en
dc.contributor.author Clover, GRG en
dc.contributor.author MacDiarmid, Robin en
dc.contributor.author Cohen, D en
dc.date.accessioned 2012-03-28T01:12:55Z en
dc.date.issued 2010-01 en
dc.identifier.citation Journal of Virological Methods 163(1):49-56 Jan 2010 en
dc.identifier.issn 0166-0934 en
dc.identifier.uri http://hdl.handle.net/2292/15763 en
dc.description.abstract Although a number of protocols have been developed for detection of viruses at the genus or family level, universal approaches to detect and identify unknown viruses are still required. High-resolution tandem mass spectrometry was used to identify accurately peptide masses and their constituent sequences from partially purified plant virus preparations. Analysis of the peptide fragment masses against a virus database using pattern-matching algorithms identified sequences with homology to known virus peptides and also predicted peptides using de novo sequence analysis. This method provided sufficient information to confirm the identity of two known viruses that were included as controls (Cucumber mosaic virus and Tomato spotted wilt virus) and to identify unknown viruses in six viral isolates. The unknown viruses have been identified as four common viruses (Alfalfa mosaic virus, Tobacco streak virus, Citrus leaf blotch virus and Ribgrass mosaic virus), and two novel viruses (a potexvirus and a vitivirus). The identification of viruses from five distinct families by the tandem mass spectrometric determination of their coat protein demonstrates that this is a useful method for initial virus identification. This method, complemented with molecular or immunological procedures, provides a rapid and convenient way to identify both known and novel plant viruses. en
dc.language EN en
dc.publisher ELSEVIER SCIENCE BV en
dc.relation.ispartofseries Journal of Virological Methods en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0166-0934/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Tandem mass spectrometry en
dc.subject Viral coat protein en
dc.subject Peptide sequencing en
dc.subject Peptide de novo sequencing en
dc.subject Virus identification en
dc.subject MOSAIC-VIRUS en
dc.subject RT-PCR en
dc.subject IDENTIFICATION en
dc.subject SEQUENCE en
dc.subject POTYVIRUSES en
dc.subject PRIMERS en
dc.subject ANTIBODIES en
dc.title A generic method to identify plant viruses by high-resolution tandem mass spectrometry of their coat proteins en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.jviromet.2009.08.009 en
pubs.issue 1 en
pubs.begin-page 49 en
pubs.volume 163 en
dc.rights.holder Copyright: Elsevier B.V. en
dc.identifier.pmid 19712699 en
pubs.end-page 56 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 118664 en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.record-created-at-source-date 2012-02-20 en
pubs.dimensions-id 19712699 en

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