Type I vs type II spiral ganglion neurons exhibit differential survival and neuritogenesis during cochlear development.

Show simple item record

dc.contributor.author Barclay, Meagan en
dc.contributor.author Ryan, AF en
dc.contributor.author Housley, GD en
dc.coverage.spatial England en
dc.date.accessioned 2012-03-28T20:35:07Z en
dc.date.issued 2011 en
dc.identifier.citation Neural Development 6:33 2011 en
dc.identifier.uri http://hdl.handle.net/2292/15831 en
dc.description.abstract Background The mechanisms that consolidate neural circuitry are a major focus of neuroscience. In the mammalian cochlea, the refinement of spiral ganglion neuron (SGN) innervation to the inner hair cells (by type I SGNs) and the outer hair cells (by type II SGNs) is accompanied by a 25% loss of SGNs. Results We investigated the segregation of neuronal loss in the mouse cochlea using β-tubulin and peripherin antisera to immunolabel all SGNs and selectively type II SGNs, respectively, and discovered that it is the type II SGN population that is predominately lost within the first postnatal week. Developmental neuronal loss has been attributed to the decline in neurotrophin expression by the target hair cells during this period, so we next examined survival of SGN sub-populations using tissue culture of the mid apex-mid turn region of neonatal mouse cochleae. In organotypic culture for 48 hours from postnatal day 1, endogenous trophic support from the organ of Corti proved sufficient to maintain all type II SGNs; however, a large proportion of type I SGNs were lost. Culture of the spiral ganglion as an explant, with removal of the organ of Corti, led to loss of the majority of both SGN sub-types. Brain-derived neurotrophic factor (BDNF) added as a supplement to the media rescued a significant proportion of the SGNs, particularly the type II SGNs, which also showed increased neuritogenesis. The known decline in BDNF production by the rodent sensory epithelium after birth is therefore a likely mediator of type II neuron apoptosis. Conclusion Our study thus indicates that BDNF supply from the organ of Corti supports consolidation of type II innervation in the neonatal mouse cochlea. In contrast, type I SGNs likely rely on additional sources for trophic support. en
dc.language eng en
dc.publisher BioMed Central Ltd. en
dc.relation.ispartofseries Neural Development en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1749-8104/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Type I vs type II spiral ganglion neurons exhibit differential survival and neuritogenesis during cochlear development. en
dc.type Journal Article en
dc.identifier.doi 10.1186/1749-8104-6-33 en
pubs.begin-page 33 en
pubs.volume 6 en
dc.rights.holder Copyright: BioMed Central Ltd. en
dc.identifier.pmid 21989106 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 285608 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Population Health en
pubs.org-id Audiology en
dc.identifier.eissn 1749-8104 en
dc.identifier.pii 1749-8104-6-33 en
pubs.record-created-at-source-date 2012-03-29 en
pubs.dimensions-id 21989106 en

Files in this item

There are no files associated with this item.

Find Full text

This item appears in the following Collection(s)

Show simple item record


Search ResearchSpace