dc.contributor.author |
Bogomolny, Evgeny |
en |
dc.contributor.author |
Huleihel, M |
en |
dc.contributor.author |
Suproun, Y |
en |
dc.contributor.author |
Sahu, R |
en |
dc.contributor.author |
Mordechai, S |
en |
dc.date.accessioned |
2012-03-29T00:44:38Z |
en |
dc.date.issued |
2007 |
en |
dc.identifier.citation |
Journal of Biomedical Optics 12(2) 2007 |
en |
dc.identifier.issn |
1083-3668 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/15907 |
en |
dc.description.abstract |
Fourier transform infrared microspectroscopy FTIR-MSP is potentially a powerful analytical method for identifying the spectral properties of biological activity in cells. The goal of the present research is the implementation of FTIR-MSP to study early spectral changes accompanying malignant transformation of cells. As a model system, cells in culture are infected by the murine sarcoma virus MuSV , which induces malignant transformation. The spectral measurements are taken at various postinfection time intervals. To follow up systematically the progress of the spectral changes at early stages of cell transformation, it is essential first to determine and validate consistent and significant spectral parameters biomarkers , which can evidently discriminate between normal and cancerous cells. Early stages of cell transformation are classified by an array of spectral biomarkers utilizing cluster analysis and discriminant classification function techniques. The classifications indicate that the first spectral changes are detectable much earlier than the first morphological signs of cell transformation. Our results point out that the first spectral signs of malignant transformation are observed on the first and third day of postinfection PI for NIH/3T3 and MEF cell cultures, respectively , while the first visible morphological alterations are observed only on the third and seventh day, respectively. These results strongly support the potential of developing FTIR microspectroscopy as a simple, reagent-free method for early detection of malignancy |
en |
dc.publisher |
Society of Photo-Optical Instrumentation Engineers (SPIE) |
en |
dc.relation.ispartofseries |
Journal of Biomedical Optics |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
Details obtained from http://www.sherpa.ac.uk/romeo/issn/1083-3668/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1117/1.2717186 |
en |
pubs.issue |
2 |
en |
pubs.begin-page |
024003 |
en |
pubs.volume |
12 |
en |
dc.rights.holder |
Copyright: Society of Photo-Optical Instrumentation Engineers (SPIE) |
en |
dc.identifier.pmid |
17477718 |
en |
pubs.end-page |
024009 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
242955 |
en |
pubs.record-created-at-source-date |
2011-11-22 |
en |
pubs.dimensions-id |
17477718 |
en |