Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy

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dc.contributor.author Bogomolny, Evgeny en
dc.contributor.author Huleihel, M en
dc.contributor.author Suproun, Y en
dc.contributor.author Sahu, R en
dc.contributor.author Mordechai, S en
dc.date.accessioned 2012-03-29T00:44:38Z en
dc.date.issued 2007 en
dc.identifier.citation Journal of Biomedical Optics 12(2) 2007 en
dc.identifier.issn 1083-3668 en
dc.identifier.uri http://hdl.handle.net/2292/15907 en
dc.description.abstract Fourier transform infrared microspectroscopy FTIR-MSP is potentially a powerful analytical method for identifying the spectral properties of biological activity in cells. The goal of the present research is the implementation of FTIR-MSP to study early spectral changes accompanying malignant transformation of cells. As a model system, cells in culture are infected by the murine sarcoma virus MuSV , which induces malignant transformation. The spectral measurements are taken at various postinfection time intervals. To follow up systematically the progress of the spectral changes at early stages of cell transformation, it is essential first to determine and validate consistent and significant spectral parameters biomarkers , which can evidently discriminate between normal and cancerous cells. Early stages of cell transformation are classified by an array of spectral biomarkers utilizing cluster analysis and discriminant classification function techniques. The classifications indicate that the first spectral changes are detectable much earlier than the first morphological signs of cell transformation. Our results point out that the first spectral signs of malignant transformation are observed on the first and third day of postinfection PI for NIH/3T3 and MEF cell cultures, respectively , while the first visible morphological alterations are observed only on the third and seventh day, respectively. These results strongly support the potential of developing FTIR microspectroscopy as a simple, reagent-free method for early detection of malignancy en
dc.publisher Society of Photo-Optical Instrumentation Engineers (SPIE) en
dc.relation.ispartofseries Journal of Biomedical Optics en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1083-3668/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Early spectral changes of cellular malignant transformation using Fourier transform infrared microspectroscopy en
dc.type Journal Article en
dc.identifier.doi 10.1117/1.2717186 en
pubs.issue 2 en
pubs.begin-page 024003 en
pubs.volume 12 en
dc.rights.holder Copyright: Society of Photo-Optical Instrumentation Engineers (SPIE) en
dc.identifier.pmid 17477718 en
pubs.end-page 024009 en
pubs.publication-status Published en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 242955 en
pubs.record-created-at-source-date 2011-11-22 en
pubs.dimensions-id 17477718 en

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