dc.contributor.author |
Dingley, Andrew |
en |
dc.contributor.author |
Nisius, L |
en |
dc.contributor.author |
Cordier, F |
en |
dc.contributor.author |
Grzesiek, S |
en |
dc.date.accessioned |
2012-03-29T19:17:15Z |
en |
dc.date.issued |
2008 |
en |
dc.identifier.citation |
Nature Protocols 3(2):242-248 2008 |
en |
dc.identifier.issn |
1750-2799 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/15993 |
en |
dc.description.abstract |
A nuclear magnetic resonance (NMR) experiment is described for the direct detection of N-H[...]N hydrogen bonds (H-bonds) in 15N isotope-labeled biomolecules. This quantitative HNN-COSY (correlation spectroscopy) experiment detects and quantifies electron-mediated scalar couplings across the H-bond (H-bond scalar couplings), which connect magnetically active (15)N nuclei of the H-bond donor and acceptor. Detectable H-bonds comprise the imino H-bonds in canonical Watson-Crick base pairs, many H-bonds in unusual nucleic acid base pairs and H-bonds between protein backbone or side-chain N-H donor and N acceptor moieties. Unlike other NMR observables, which provide only indirect evidence of the presence of H-bonds, the H-bond scalar couplings identify all partners of the H-bond, the donor, the donor proton and the acceptor in a single experiment. The size of the scalar couplings can be related to H-bond geometries and as a time average to H-bond dynamics. The time required to detect the H-bonds is typically less than 1 d at millimolar concentrations for samples of molecular weight < or = approximately 25 kDa. A C15N/13C-labeled potato spindle tuber viroid T1 RNA domain is used as an example to illustrate this procedure. |
en |
dc.description.uri |
http://www.nature.com/nprot/journal/v3/n2/abs/nprot.2007.497.html |
en |
dc.publisher |
Nature Publishing Group |
en |
dc.relation.ispartofseries |
Nature Protocols |
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dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.
Details obtained from http://www.sherpa.ac.uk/romeo/issn/1754-2189/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Direct detection of N-H⋯N hydrogen bonds in biomolecules by NMR spectroscopy |
en |
dc.type |
Journal Article |
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pubs.issue |
2 |
en |
pubs.begin-page |
242 |
en |
pubs.volume |
3 |
en |
dc.rights.holder |
Copyright: Nature Publishing Group |
en |
pubs.end-page |
248 |
en |
pubs.publication-status |
Published |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
84301 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
18274526 |
en |