A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.

A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.

Baker, Daniel ; May, C ; Brauner, CJ
Identifier: http://hdl.handle.net/2292/16037
Issue Date: 2009
Reference: JOURNAL OF FISH BIOLOGY 75(1):268-275 2009
Rights: Copyright: The Fisheries Society of the British Isles; The authors
Rights (URI): https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm

Abstract:

This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).

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DOI: 10.1111/j.1095-8649.2009.02261.x
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