A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.

Baker, Daniel; May, C; Brauner, CJ

dc.contributor.author	Baker, Daniel	en
dc.contributor.author	May, C	en
dc.contributor.author	Brauner, CJ	en
dc.coverage.spatial	England	en
dc.date.accessioned	2012-03-29T19:22:43Z	en
dc.date.issued	2009	en
dc.identifier.citation	JOURNAL OF FISH BIOLOGY 75(1):268-275 2009	en
dc.identifier.uri	http://hdl.handle.net/2292/16037	en
dc.description.abstract	This study assessed the effect of tissue storage duration and accuracy of the metabolic inhibitor tissue homogenate (MITH) method on intracellular pH (pHi) values of tissues of white sturgeon Acipenser transmontanus during hypercarbia. No effect of storage in liquid nitrogen of up to 30 days was observed and MITH appears appropriate for measurement of pH in fish exposed to up to 10% CO2 (10000 Pa pCO2).	en
dc.language	eng	en
dc.publisher	The Fisheries Society of the British Isles; The Authors	en
dc.relation.ispartofseries	Journal of Fish Biology	en
dc.rights	Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher.	en
dc.rights.uri	https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm	en
dc.subject	Acidosis	en
dc.subject	Animals	en
dc.subject	Carbon Dioxide	en
dc.subject	Erythrocytes	en
dc.subject	Fish Diseases	en
dc.subject	Fishes	en
dc.subject	Freezing	en
dc.subject	Hydrogen-Ion Concentration	en
dc.subject	Intracellular Fluid	en
dc.subject	Reproducibility of Results	en
dc.subject	Specimen Handling	en
dc.subject	Time Factors	en
dc.subject	Zoology	en
dc.title	A validation of intracellular pH measurements in fish exposed to hypercarbia: the effect of duration of tissue storage and efficacy of the metabolic inhibitor tissue homogenate method.	en
dc.type	Journal Article	en
dc.identifier.doi	10.1111/j.1095-8649.2009.02261.x	en
pubs.issue	1	en
pubs.begin-page	268	en
pubs.volume	75	en
dc.rights.holder	Copyright: The Fisheries Society of the British Isles; The authors	en
dc.identifier.pmid	20738495	en
pubs.end-page	275	en
dc.rights.accessrights	http://purl.org/eprint/accessRights/RestrictedAccess	en
pubs.subtype	Article	en
pubs.subtype	Rapid Communication	en
pubs.elements-id	230487	en
dc.identifier.eissn	1095-8649	en
dc.identifier.pii	JFB2261	en
pubs.record-created-at-source-date	2011-10-12	en
pubs.dimensions-id	20738495	en