An in vitro model of human placental trophoblast deportation/shedding.

Show simple item record Abumaree, M en Stone, Peter en Chamley, Lawrence en 2012-03-29T20:53:40Z en 2006 en
dc.identifier.citation Molecular Human Reproduction 12(11):687-694 Nov 2006 en
dc.identifier.issn 1360-9947 en
dc.identifier.uri en
dc.description.abstract Deportation of trophoblast shed from the placenta into the maternal circulation was first described over 100 years ago. Despite this, little is known about the quantity or nature of the shed and deported trophoblasts. Neither do we have a clear understanding of the fate of deported trophoblasts nor do we have a clear understanding of their effects on the maternal physiology. This deficiency is largely due to the inaccessibility of deported trophoblasts in vivo. This study aimed to produce a model that would allow us to study deported trophoblasts. We devised a system for culturing placental explants of 12-week gestation in cell culture inserts with a stainless steel mesh bottom that allowed the ready harvesting of shed/deported trophoblasts. Immunohistochemical and morphologic investigations demonstrated that these in vitro shed/deported trophoblasts are similar to those found in vivo and that apoptotic, necrotic and viable trophoblasts were shed from the explants. Inhibiting caspases induced a change from predominantly apoptotic to predominantly necrotic trophoblast shedding. We have devised an in vitro model that allows the collection of shed/deported trophoblasts which will significantly enhance our ability to study these cells. Our preliminary investigations confirm that apoptosis plays an important role in trophoblast shedding/deportation. en
dc.publisher Oxford University Press en
dc.relation.ispartofseries Molecular Human Reproduction en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from: en
dc.rights.uri en
dc.title An in vitro model of human placental trophoblast deportation/shedding. en
dc.type Journal Article en
dc.identifier.doi 10.1093/molehr/gal073 en
pubs.begin-page 687 en
pubs.volume 12 en
dc.rights.holder Copyright: Oxford University Press en
dc.identifier.pmid 16988002 en
pubs.end-page 694 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 96848 en Medical and Health Sciences en School of Medicine en Obstetrics and Gynaecology en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 16988002 en

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