Studies on cytotoxic lymphocyte production in culture

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dc.contributor.advisor Marbrook, J. en
dc.contributor.author Christensen, Neil D. en
dc.date.accessioned 2007-08-29T06:54:41Z en
dc.date.available 2007-08-29T06:54:41Z en
dc.date.issued 1983 en
dc.identifier THESIS 84-021 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1983 en
dc.identifier.uri http://hdl.handle.net/2292/1611 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract Clones of cytotoxic T lymphocytes (CTL) responding to the hapten fluorescein have been examined by limiting dilution analysis. The analysis was carried out to determine the frequency of anti-fluorescein CTL precursor cells, the size of clones of cytotoxic cells, and the specificity of the cytotoxicity generated. Two limiting dilution culture procedures were developed to examine both the appearance of clones of anti-fluorescein CTL with time, and the stages in the CTL developmental pathway. 1. Limiting dilution cultures of normal spleen cells that received growth factors and stimulator cells at the start of the culture period. The precursor cell frequency reached a maximum value on days 4 to 6 at 1 precursor in 8-10,000 spleen cells in this system. 2. Limiting dilution cultures of normal spleen cells that received growth factors and stimulator cells on both Days 0 and 3 of the culture period. The precursor cell frequency reached a maximum value of 1 in 1000 spleen cells later in the response on Days 7 to 9 in this restimulated system. The precursor cells giving rise to clones of CTL on Day 5, and those giving rise to clones of CTL on Day 7 were interpreted as belonging to two subpopulations of precursor cells. The possibility that these two subpopulations represented separate stages of the anti-fluorescein CTL developmental pathway was suggested. The specificity of the cytotoxicity generated in restimulated cultures was dependent on both the source and quantity of growth factors. When high concentrations of untreated growth factors obtained from rat spleen cell cultures were added, non-specific cytotoxicity was generated. When the growth factors were semi-purified by size-column and ion-exchange column chromatography, highly specific anti-fluorescein cytotoxicity was generated. Non-specific growth factors other than Interleukin 2 were found to be required for the production of anti-fluorescein CTL. en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA9921940214002091 en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Studies on cytotoxic lymphocyte production in culture en
dc.type Thesis en
thesis.degree.discipline Cell Biology en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.rights.holder Copyright: The author en


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