Distribution of fucosylated xyloglucans among the walls of different cell types in monocotyledons determined by immunofluorescence microscopy

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dc.contributor.author Harris, Philip en
dc.contributor.author Brennan, M en
dc.date.accessioned 2012-03-29T22:41:22Z en
dc.date.issued 2011 en
dc.identifier.citation Molecular Plant 4(1):144-156 2011 en
dc.identifier.issn 1674-2052 en
dc.identifier.uri http://hdl.handle.net/2292/16131 en
dc.description.abstract Xyloglucans in the non-lignified primary cell walls of different species of monocotyledons have diverse structures, with widely varying proportions of oligosaccharide units that contain fucosylated side chains (F side chains). To determine whether fucosylated xyloglucans occur in all non-lignified walls in a range of monocotyledon species, we used immunofluorescence microscopy with the monoclonal antibody CCRC-M1. The epitope of this antibody, α-L-Fucp-(1→2)-β-D-Galp, occurs in F side chains. In most non-commelinid monocotyledons, the epitope was found in all non-lignified walls. A similar distribution was found in the palm Phoenix canariensis, which is a member of the basal commelinid order Arecales. However, in the other commelinid orders Zingiberales, Commelinales, and Poales, the occurrence of the epitope was restricted, sometimes occurring in only the phloem walls, but often also in walls of other cell types including stomatal guard and subsidiary cells and raphide idioblasts. No epitope was found in the walls of the commelinids Tradescantia virginiana (Commelinaceae, Commelinales) and Zea mays (Poaceae, Poales), but it occurred in the phloem walls of two other Poaceae species, Lolium multiflorum and L. perenne. The distribution of the epitope is discussed in relation to xyloglucan structures in the different taxa. However, the functional significance of the restricted distributions is unknown. en
dc.publisher The Molecular Plant Shanghai Editorial Office in association with Oxford University Press on behalf of CSPP and IPPE, SIBS, CAS. en
dc.relation.ispartofseries Molecular Plant en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1674-2052/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Distribution of fucosylated xyloglucans among the walls of different cell types in monocotyledons determined by immunofluorescence microscopy en
dc.type Journal Article en
dc.identifier.doi 10.1093/mp/ssq067 en
pubs.issue 1 en
pubs.begin-page 144 en
pubs.volume 4 en
dc.rights.holder Copyright: the authors en
dc.identifier.pmid 20978085 en
pubs.end-page 156 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 188545 en
pubs.record-created-at-source-date 2010-11-30 en
pubs.dimensions-id 20978085 en

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