dc.contributor.author |
Rosenberg, MF |
en |
dc.contributor.author |
O'Ryan, Liam |
en |
dc.contributor.author |
Hughes, G |
en |
dc.contributor.author |
Zhao, Z |
en |
dc.contributor.author |
Aleksandrov, LA |
en |
dc.contributor.author |
Riordan, JR |
en |
dc.contributor.author |
Ford, RC |
en |
dc.date.accessioned |
2012-04-02T20:17:21Z |
en |
dc.date.issued |
2011 |
en |
dc.identifier.citation |
Journal of Biological Chemistry 42647-42654 2011 |
en |
dc.identifier.issn |
0021-9258 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/16414 |
en |
dc.description.abstract |
Cystic fibrosis affects about 1 in 2500 live births and involves loss of transmembrane chloride flux due to a lack of a membrane protein channel termed the cystic fibrosis transmembrane conductance regulator (CFTR). We have studied CFTR structure by electron crystallography. The data were compared with existing structures of other ATP-binding cassette transporters. The protein was crystallized in the outward facing state and resembled the well characterized Sav1866 transporter. We identified regions in the CFTR map, not accounted for by Sav1866, which were potential locations for the regulatory region as well as the channel gate. In this analysis, we were aided by the fact that the unit cell was composed of two molecules not related by crystallographic symmetry. We also identified regions in the fitted Sav1866 model that were missing from the map, hence regions that were either disordered in CFTR or differently organized compared with Sav1866. Apart from the N and C termini, this indicated that in CFTR, the cytoplasmic end of transmembrane helix 5/11 and its associated loop could be partly disordered (or alternatively located). |
en |
dc.publisher |
American Society for Biochemistry and Molecular Biology |
en |
dc.relation.ispartofseries |
Journal of Biological Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0021-9258/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
The Cystic Fibrosis Transmembrane Conductance Regulator (CFTR): three-dimensional structure and localization of a channel gate. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1074/jbc.M111.292268 |
en |
pubs.begin-page |
42647 |
en |
dc.rights.holder |
Copyright: American Society for Biochemistry and Molecular Biology |
en |
dc.identifier.pmid |
21931164 |
en |
pubs.end-page |
42654 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
324083 |
en |
pubs.record-created-at-source-date |
2012-03-16 |
en |
pubs.dimensions-id |
21931164 |
en |