Replication from the primary origin of the mini-F plasmid

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dc.contributor.advisor Bergquist, P. en
dc.contributor.advisor Lane, D. en
dc.contributor.author Caughey, Penelope A. en
dc.date.accessioned 2007-09-03T09:26:40Z en
dc.date.available 2007-09-03T09:26:40Z en
dc.date.issued 1986 en
dc.identifier THESIS 87-014 en
dc.identifier.citation Thesis (PhD--Cell Biology)--University of Auckland, 1986 en
dc.identifier.uri http://hdl.handle.net/2292/1673 en
dc.description Full text is available to authenticated members of The University of Auckland only. en
dc.description.abstract The work in this thesis concerns the analysis of essential functions required for replication initiation from origin -1 of the mini-F replicon of F plasmid. The techniques principally employed were the cloning of restriction fragments, DNA sequencing and sequence analyses, assays for replicative ability in DNA polymerase 1 - dependent hosts, protein purification techniques and protein – DNA binding. This work led to the following findings - 1. A 198 base pair region of mini-F contains the sequence required for initiation of replication from origin 1 in a DNA polymerase 1 -dependent host. 2. The mini-F encoded proteins C, G and E are required for replication from this origin. 3. The C protein is a 38,739 dalton protein which binds in vitro to two DNA regions - one contains the operator of the C gene and the other region contains the minimal origin sequence. en
dc.language.iso en en
dc.publisher ResearchSpace@Auckland en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.relation.isreferencedby UoA9910169114002091 en
dc.rights Restricted Item. Available to authenticated members of The University of Auckland. en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Replication from the primary origin of the mini-F plasmid en
dc.type Thesis en
thesis.degree.discipline Cell Biology en
thesis.degree.grantor The University of Auckland en
thesis.degree.level Doctoral en
thesis.degree.name PhD en
dc.rights.holder Copyright: The author en


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