dc.contributor.author |
Kalev, Maggie |
en |
dc.contributor.author |
Symes, W |
en |
dc.contributor.author |
Young, Deborah |
en |
dc.contributor.author |
During, Matthew |
en |
dc.date.accessioned |
2012-04-05T01:44:50Z |
en |
dc.date.issued |
2009 |
en |
dc.identifier.citation |
Molecular and Cellular Neuroscience 41(4):383-396 2009 |
en |
dc.identifier.issn |
1044-7431 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/16828 |
en |
dc.description.abstract |
The N-methyl-d-aspartate receptor (NMDAR) is critically involved in learning and memory, neuronal survival, as well as neuroexcitotoxicity and seizures. We hypothesize that even mild reductions in the numbers of hippocampal NMDARs could impair learning and memory, whereas increasing receptor activity would facilitate learning but reduce seizure threshold. We developed novel gene transfer strategies assisted by an adeno-associated viral vector 1/2 to bi-directionally modulate expression levels of the NR1 protein in rat hippocampus. Functional consequences of the altered NR1 expression were examined in the acute seizure model, and on normal processes of fear memory and neurogenesis. We found that knocking down NR1 protected against seizures at the expense of impaired learning, as predicted. Paradoxically, NR1 overexpression not only increased fear memory and neurogenesis, but also delayed onset of more severe seizures. In conclusion, the observed consequences of NR1 knockdown and overexpression underscore NMDAR requirement for neuronal plasticity, and are in agreement with its dichotomous functions. |
en |
dc.publisher |
Elsevier Inc |
en |
dc.relation.ispartofseries |
Molecular and Cellular Neuroscience |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1044-7431/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Knockdown and overexpression of NR1 modulates NMDA receptor function. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1016/j.mcn.2009.04.003 |
en |
pubs.issue |
4 |
en |
pubs.begin-page |
383 |
en |
pubs.volume |
41 |
en |
dc.rights.holder |
Copyright: Elsevier Inc |
en |
dc.identifier.pmid |
19394426 |
en |
pubs.end-page |
396 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
95234 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacology |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
19394426 |
en |