dc.contributor.author |
Kanwar, JR |
en |
dc.contributor.author |
Palmano, KP |
en |
dc.contributor.author |
Sun, Xueying |
en |
dc.contributor.author |
Kanwar, RK |
en |
dc.contributor.author |
Gupta, R |
en |
dc.contributor.author |
Haggarty, N |
en |
dc.contributor.author |
Rowan, A |
en |
dc.contributor.author |
Ram, S |
en |
dc.contributor.author |
Krissansen, Geoffrey |
en |
dc.coverage.spatial |
Australia |
en |
dc.date.accessioned |
2012-04-18T00:23:44Z |
en |
dc.date.issued |
2008 |
en |
dc.identifier.citation |
Immunology and Cell Biology 86(3):277-288 2008 |
en |
dc.identifier.issn |
0818-9641 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/17362 |
en |
dc.description.abstract |
Bovine lactoferrin (bLf), an iron-containing natural defence protein found in bodily secretions, has been reported to inhibit carcinogenesis and the growth of tumours. Here, we investigated whether natural bLf and iron-saturated forms of bLf differ in their ability to augment cancer chemotherapy. bLf was supplemented into the diet of C57BL/6 mice that were subsequently challenged subcutaneously with tumour cells, and treated by chemotherapy. Chemotherapy eradicated large (0.6 cm diameter) EL-4 lymphomas in mice that had been fed iron-saturated bLf (here designated Lf(+)) for 6 weeks prior to chemotherapy, but surprisingly not in mice that were fed lesser iron-saturated forms of bLf, including apo-bLf (4% iron saturated), natural bLf (approximately 15% iron saturated) and 50% iron-saturated bLf. Lf(+)-fed mice bearing either EL-4, Lewis lung carcinoma or B16 melanoma tumours completely rejected their tumours within 3 weeks following a single injection of either paclitaxel, doxorubicin, epirubicin or fluorouracil, whereas mice fed the control diet were resistant to chemotherapy. Lf(+) had to be fed to mice for more than 2 weeks prior to chemotherapy to be wholly effective in eradicating tumours from all mice, suggesting that it acts as a competence factor. It significantly reduced tumour vascularity and blood flow, and increased antitumour cytotoxicity, tumour apoptosis and the infiltration of tumours by leukocytes. Lf(+) bound to the intestinal epithelium and was preferentially taken up within Peyer's patches. It increased the production of Th1 and Th2 cytokines within the intestine and tumour, including TNF, IFN-gamma, as well as nitric oxide that have been reported to sensitize tumours to chemotherapy. Importantly, it restored both red and white peripheral blood cell numbers depleted by chemotherapy, potentially fortifying the mice against cancer. In summary, bLf is a potent natural adjuvant and fortifying agent for augmenting cancer chemotherapy, but needs to be saturated with iron to be effective. |
en |
dc.language |
eng |
en |
dc.publisher |
Nature Publishing Group |
en |
dc.relation.ispartofseries |
Immunology and Cell Biology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0818-9641/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Adjuvants, Immunologic |
en |
dc.subject |
Adjuvants, Pharmaceutic |
en |
dc.subject |
Animals |
en |
dc.subject |
Antineoplastic Combined Chemotherapy Protocols |
en |
dc.subject |
Carcinoma, Lewis Lung |
en |
dc.subject |
Cattle |
en |
dc.subject |
Cytotoxicity, Immunologic |
en |
dc.subject |
Dietary Supplements |
en |
dc.subject |
Drug Resistance, Neoplasm |
en |
dc.subject |
Immunohistochemistry |
en |
dc.subject |
Iron |
en |
dc.subject |
Lactoferrin |
en |
dc.subject |
Lymphoma |
en |
dc.subject |
Melanoma, Experimental |
en |
dc.subject |
Mice |
en |
dc.subject |
Mice, Inbred C57BL |
en |
dc.subject |
Neoplasm Transplantation |
en |
dc.subject |
Neovascularization, Pathologic |
en |
dc.title |
'Iron-saturated' lactoferrin is a potent natural adjuvant for augmenting cancer chemotherapy. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1038/sj.icb.7100163 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
277 |
en |
pubs.volume |
86 |
en |
dc.rights.holder |
Copyright: Australasian Society for Immunology Inc. |
en |
dc.identifier.pmid |
18268518 |
en |
pubs.end-page |
288 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
115063 |
en |
dc.identifier.pii |
7100163 |
en |
pubs.record-created-at-source-date |
2012-04-18 |
en |
pubs.dimensions-id |
18268518 |
en |