dc.contributor.author |
Hull, ML |
en |
dc.contributor.author |
Escareno, CR |
en |
dc.contributor.author |
Godsland, JM |
en |
dc.contributor.author |
Doig, JR |
en |
dc.contributor.author |
Johnson, CM |
en |
dc.contributor.author |
Phillips, SC |
en |
dc.contributor.author |
Smith, SK |
en |
dc.contributor.author |
Tavare, S |
en |
dc.contributor.author |
Print, Cristin |
en |
dc.contributor.author |
Charnock-Jones, DS |
en |
dc.date.accessioned |
2012-05-13T20:26:18Z |
en |
dc.date.issued |
2008-09-01 |
en |
dc.identifier.citation |
AMERICAN JOURNAL OF PATHOLOGY 173(3):700-715 01 Sep 2008 |
en |
dc.identifier.issn |
0002-9440 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/17921 |
en |
dc.description.abstract |
The pathophysiology of endometriosis remains unclear but involves a complex interaction between ectopic endometrium and host peritoneal tissues. We hypothesized that disruption of this interaction would suppress endometriotic lesion formation. We hoped to delineate the molecular and cellular dialogue between ectopic human endometrium and peritoneal tissues in nude mice as a first step toward testing this hypothesis. Human endometrium was xenografted into nude mice, and the resulting lesions were analyzed using microarrays. A novel technique was developed that unambiguously determined whether RNA transcripts identified via microarray analyses originated from human cells (endometrium) or mouse cells (mesothelium). Four key pathways (ubiquitin/proteasome, inflammation, tissue remodeling/repair, and ras-mediated oncogenesis) were revealed, demonstrating communication between host mesothelial cells and ectopic endometrium. Morphometric analysis of nude mouse lesions confirmed that necrosis, inflammation, healing and repair, and cell proliferation occurred during xenograft development. These processes were entirely consistent with the molecular networks revealed by the microarray data. The transcripts detected in the xenografts; overlapped with differentially expressed transcripts in a comparison between paired eutopic and ectopic endometria from human endometriotic patients. For the first time, components of the interaction between ectopic endometrium and peritoneal stromal tissues are revealed. Targeted disruption of this dialogue is likely to inhibit endometriotic tissue formation and may prove to be an effective therapeutic strategy for endometriosis. |
en |
dc.language |
English |
en |
dc.publisher |
Elsevier; American Society for Investigative Pathology (ASIP) |
en |
dc.relation.ispartofseries |
American Journal of Pathology |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0002-9440/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Life Sciences & Biomedicine |
en |
dc.subject |
Pathology |
en |
dc.subject |
ENDOTHELIAL GROWTH-FACTOR |
en |
dc.subject |
FACTOR-KAPPA-B |
en |
dc.subject |
GENE-EXPRESSION |
en |
dc.subject |
EUTOPIC ENDOMETRIUM |
en |
dc.subject |
NUDE-MICE |
en |
dc.subject |
ECTOPIC ENDOMETRIUM |
en |
dc.subject |
MICROARRAY ANALYSIS |
en |
dc.subject |
OVARIAN-STEROIDS |
en |
dc.subject |
HUMAN-DISEASE |
en |
dc.subject |
MOUSE MODEL |
en |
dc.title |
Endometrial-peritoneal interactions during endometriotic lesion establishment |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.2353/ajpath.2008.071128 |
en |
pubs.issue |
3 |
en |
pubs.begin-page |
700 |
en |
pubs.volume |
173 |
en |
dc.rights.holder |
Copyright: Elsevier; American Society for Investigative Pathology (ASIP) |
en |
dc.identifier.pmid |
18688027 |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000258921900010&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.end-page |
715 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
115846 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Science Research |
en |
pubs.org-id |
Maurice Wilkins Centre (2010-2014) |
en |
pubs.record-created-at-source-date |
2012-05-14 |
en |
pubs.dimensions-id |
18688027 |
en |