A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4.

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dc.contributor.author Reynolds, Clare en
dc.contributor.author Draper, E en
dc.contributor.author Keogh, B en
dc.contributor.author Rahman, A en
dc.contributor.author Moloney, AP en
dc.contributor.author Mills, KH en
dc.contributor.author Loscher, CE en
dc.contributor.author Roche, HM en
dc.coverage.spatial United States en
dc.date.accessioned 2012-05-13T20:36:30Z en
dc.date.issued 2009-12 en
dc.identifier.citation The Journal of Nutrition 139(12):2351-2357 Dec 2009 en
dc.identifier.issn 0022-3166 en
dc.identifier.uri http://hdl.handle.net/2292/17924 en
dc.description.abstract Conjugated linoleic acid (CLA) is a PUFA found in beef and dairy products that has immunoregulatory properties. The level of CLA in beef can be enhanced by feeding cattle fresh grass rather than concentrates. This study determined the effect of feeding a high-CLA beef diet on inflammation in an in vivo model of septic shock. Mice were fed a high-CLA beef (4.3% total fatty acid composition) or low-CLA beef diet (0.84% total fatty acid composition) for 6 wk. Lipopolysaccharide (LPS; 3 microg) or sterile PBS was injected i.v. and serum was harvested 6 h after injection. Serum interleukin (IL)-1beta, IL-12p70, IL-12p40, and interferon-gamma concentrations were significantly reduced in response to the LPS challenge in the high-CLA beef diet group. Bone marrow-derived dendritic cells (BMDC) from the high-CLA beef diet group had significantly less IL-12 and more IL-10 in response to ex vivo LPS stimulation. Furthermore, toll-like receptor 4 (TLR4) and CD14 protein and mRNA expression on BMDC was significantly attenuated in the high-CLA compared with the low-CLA beef diet group. Complimentary in vitro experiments to determine the specificity of the effect showed that synthetic cis9, trans11-CLA suppressed surface expression of CD14 and TLR4 on BMDC. Treatment with the PPARgamma inhibitor GW9662 partially reversed TLR4 expression in immature BMDC. The results of this study demonstrate that feeding a diet enriched in high-beef CLA exerts profound antiinflammatory effects in vivo within the context of LPS-induced sepsis. In addition, downregulation of BMDC TLR4 is mediated through induction of PPARgamma. en
dc.language eng en
dc.publisher American Society for Nutrition en
dc.relation.ispartofseries Journal of Nutrition en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0022-3166/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.subject Animals en
dc.subject Bone Marrow en
dc.subject Cattle en
dc.subject Dendritic Cells en
dc.subject Down-Regulation en
dc.subject Female en
dc.subject Food Irradiation en
dc.subject Gene Expression Regulation en
dc.subject Inflammation en
dc.subject Linoleic Acids, Conjugated en
dc.subject Lipopolysaccharides en
dc.subject Male en
dc.subject Meat en
dc.subject Mice en
dc.subject Mice, Inbred BALB C en
dc.subject PPAR gamma en
dc.subject RNA, Messenger en
dc.subject Sepsis en
dc.subject Toll-Like Receptor 4 en
dc.title A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4. en
dc.type Journal Article en
dc.identifier.doi 10.3945/jn.109.113035 en
pubs.issue 12 en
pubs.begin-page 2351 en
pubs.volume 139 en
dc.rights.holder Copyright: American Society for Nutrition en
dc.identifier.pmid 19846417 en
pubs.end-page 2357 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 341387 en
pubs.org-id Liggins Institute en
dc.identifier.eissn 1541-6100 en
dc.identifier.pii jn.109.113035 en
pubs.record-created-at-source-date 2012-05-14 en
pubs.dimensions-id 19846417 en

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