dc.contributor.author |
Hilditch, L |
en |
dc.contributor.author |
Matadeen, R |
en |
dc.contributor.author |
Goldstone, David |
en |
dc.contributor.author |
Rosenthal, PB |
en |
dc.contributor.author |
Taylor, IA |
en |
dc.contributor.author |
Stoye, JP |
en |
dc.date.accessioned |
2012-05-13T21:19:35Z |
en |
dc.date.issued |
2011-04-05 |
en |
dc.identifier.citation |
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 108(14):5771-5776 05 Apr 2011 |
en |
dc.identifier.issn |
0027-8424 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/17928 |
en |
dc.description.abstract |
The restriction factor Fv1 confers resistance to murine leukemia virus (MLV), blocking progression of the viral life cycle after reverse transcription, but before integration into the host chromosome. It is known that the specificity of restriction is determined by both the restriction factor and the viral capsid (CA), but a direct interaction between Fv1 and MLV CA has not yet been demonstrated. With the development of a previously unexplored method for in vitro polymerization of MLV CA, it has now been possible to display a binding interaction between Fv1 and MLV CA. C-terminally His-tagged CA molecules were assembled on Ni-chelating lipid nanotubes, and analysis by electron microscopy revealed the formation of a regular lattice. Comparison of binding data with existing restriction data confirmed the specificity of the binding interaction, with multiple positions of both Fv1 and CA shown to influence binding specificity. |
en |
dc.language |
English |
en |
dc.publisher |
National Academy of Sciences |
en |
dc.relation.ispartofseries |
Proceedings of the National Academy of Sciences of the United States of America |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1091-6490/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.subject |
Science & Technology |
en |
dc.subject |
Multidisciplinary Sciences |
en |
dc.subject |
Science & Technology - Other Topics |
en |
dc.subject |
HOST RESTRICTION |
en |
dc.subject |
GENE |
en |
dc.subject |
RETROVIRUS |
en |
dc.subject |
CORE |
en |
dc.subject |
GAG |
en |
dc.subject |
DETERMINANTS |
en |
dc.subject |
TROPISM |
en |
dc.subject |
DOMAIN |
en |
dc.subject |
NB |
en |
dc.subject |
IDENTIFICATION |
en |
dc.title |
Ordered assembly of murine leukemia virus capsid protein on lipid nanotubes directs specific binding by the restriction factor, Fv1 |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1073/pnas.1100118108 |
en |
pubs.issue |
14 |
en |
pubs.begin-page |
5771 |
en |
pubs.volume |
108 |
en |
dc.rights.holder |
Copyright: National Academy of Sciences |
en |
dc.identifier.pmid |
21436027 |
en |
pubs.author-url |
http://gateway.webofknowledge.com/gateway/Gateway.cgi?GWVersion=2&SrcApp=PARTNER_APP&SrcAuth=LinksAMR&KeyUT=000289265300055&DestLinkType=FullRecord&DestApp=ALL_WOS&UsrCustomerID=6e41486220adb198d0efde5a3b153e7d |
en |
pubs.end-page |
5776 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
285409 |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.record-created-at-source-date |
2012-05-14 |
en |
pubs.dimensions-id |
21436027 |
en |