Studies on the structure of membrane bound and membrane associated proteins using scattering

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Show simple item record O'Ryan, Liam en 2012-05-13T22:52:46Z en 2009 en 2009 en
dc.identifier.citation Sub type: PhD Thesis. University of Manchester, 2009 en
dc.identifier.uri en
dc.description.abstract Membrane proteins are poorly represented in the structural databases, despite the fact that approximately 30% of all open reading frames code for them. Investigations into the structure of three membrane proteins are reported in this thesis: OpcA, the outer membrane adhesin from the pathogenic bacterium Neisseria meningitidis; Kir2.1, the inwardly rectifying potassium channel from Mus musculus; PSD95, the post-synaptic density scaffold protein from Rattus norvegicus. Recombinant OpcA was isolated from a bacterial expression system, refolded from inclusion bodies and purified in the presence of detergent. The purified protein was used in several structure determination experiments: OpcA was found to adopt a micelle sharing dimeric state in solution by small angle X-ray scattering; the location of detergent within crystals of the protein was explored by low-resolution neutron crystallography; and ligand binding was investigated by high resolution X-ray crystallography experiments. Perdeuterated OpcA was also purified and crystallised, and was found to diffract neutrons during a test scan. Finally, the structures of Kir2.1 and PSD95 were investigated by small angle scattering. A cytoplasmic domain construct of Kir2.1 was found to be have a tetrameric structure. Rigid body modelling was performed on PSD95 using the experimental small angle scattering curve to fit the domains of the protein. en
dc.publisher University of Manchester en
dc.relation.ispartof PhD Thesis - University of Auckland en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title Studies on the structure of membrane bound and membrane associated proteins using scattering en
dc.type Thesis en University of Manchester en Doctoral en PhD en
dc.rights.holder Copyright: The author en en
dc.rights.accessrights en
pubs.elements-id 328497 en
pubs.record-created-at-source-date 2012-03-20 en

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