Pulmonary fibroblasts from COPD patients show an impaired response of elastin synthesis to TGF-β1

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dc.contributor.author Zhang, J en
dc.contributor.author Wu, L en
dc.contributor.author Feng, MX en
dc.contributor.author Sexton, Paul en
dc.contributor.author Bai, CX en
dc.contributor.author Qu, JM en
dc.contributor.author Merrilees, M en
dc.contributor.author Black, PN en
dc.contributor.editor Scheid, P en
dc.date.accessioned 2012-05-27T20:56:05Z en
dc.date.issued 2011 en
dc.identifier.citation Respiratory Physiology & Neurobiology 177(3):236-240 2011 en
dc.identifier.issn 1569-9048 en
dc.identifier.uri http://hdl.handle.net/2292/18607 en
dc.description.abstract Insufficiency of tissue repair by pulmonary fibroblasts may contribute to the decrease in elastic fibres in chronic obstructive pulmonary disease (COPD). In this study, the repair function of COPD fibroblasts was assessed by examining the response to transforming growth factor (TGF)-β1. Primary pulmonary fibroblasts were cultured from lung tissue of COPD patients and smoking control subjects. Cellular proliferation was measured with Alamar Blue reduction method. Levels of tropoelastin mRNA and soluble elastin was measured using real-time RT-PCR and Fastin elastin assay respectively. The percentage of increase in proliferation and elastin production after TGF-β1 (1 ng/ml) treatment was calculated for fibroblasts from each subject. COPD fibroblasts showed slower proliferation than control fibroblasts, and a reduced response to TGF-β1 stimulation. The promotive effect of TGF-β1 on elastin synthesis in control fibroblasts was significantly diminished in fibroblasts from COPD patients. Our findings indicate that COPD lung fibroblasts have a significantly decreased response to TGF-β1 in terms of proliferation and elastin production. en
dc.publisher Elsevier en
dc.relation.ispartofseries Respiratory Physiology & Neurobiology en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/1569-9048/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Pulmonary fibroblasts from COPD patients show an impaired response of elastin synthesis to TGF-β1 en
dc.type Journal Article en
dc.identifier.doi 10.1016/j.resp.2011.04.019 en
pubs.issue 3 en
pubs.begin-page 236 en
pubs.volume 177 en
dc.rights.holder Copyright: Elsevier en
pubs.end-page 240 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 341787 en
pubs.record-created-at-source-date 2012-04-06 en


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