dc.contributor.author |
Graham, Euan |
en |
dc.contributor.author |
Ball, N |
en |
dc.contributor.author |
Scotter, Emma |
en |
dc.contributor.author |
Narayan, Pritika |
en |
dc.contributor.author |
Dragunow, Michael |
en |
dc.contributor.author |
Glass, Michelle |
en |
dc.date.accessioned |
2012-05-30T04:20:17Z |
en |
dc.date.issued |
2006 |
en |
dc.identifier.citation |
Journal of Biological Chemistry 281(39):29085-29095 2006 |
en |
dc.identifier.issn |
0021-9258 |
en |
dc.identifier.uri |
http://hdl.handle.net/2292/18788 |
en |
dc.description.abstract |
Neuro2a cells endogenously express cannabinoid type 1 (CB1) receptors. CB1 stimulation with HU210 activated ERK and induced the transcription factor Krox-24. A functional MEK-ERK pathway is an important requirement for CB1-mediated Krox-24 induction as blockade of MEK signaling by UO126 reduces both basal and CB1-mediated activation of Krox-24. CB1 receptor stimulation did not activate either JNK or p38 MAPK pathways or the pro-proliferation phosphatidylinositol 3-kinase (PI3K)-Akt pathway. However, serum removal or blockade of PI3K signaling by LY294002 transiently stimulated basal Krox-24 expression and increased CB1-mediated induction of Krox-24. This was consistent with a transient increase in pMEK, pERK, and pCREB levels following PI3K blockade. These data demonstrate that CB1-mediated activation of the Krox-24 transcription factor is negatively regulated through the PI3K-Akt pathway and reveals several points of signaling cross-talk between these two important kinase pathways. |
en |
dc.publisher |
The American Society for Biochemistry and Molecular Biology, Inc. |
en |
dc.relation.ispartofseries |
Journal of Biological Chemistry |
en |
dc.rights |
Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0021-9258/ |
en |
dc.rights.uri |
https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm |
en |
dc.title |
Induction of Krox-24 by endogenous cannabinoid type 1 receptors in Neuro2A cells is mediated by the MEK-ERK MAPK pathway and is suppressed by the phosphatidylinositol 3-kinase pathway. |
en |
dc.type |
Journal Article |
en |
dc.identifier.doi |
10.1074/jbc.M602516200 |
en |
pubs.issue |
39 |
en |
pubs.begin-page |
29085 |
en |
pubs.volume |
281 |
en |
dc.rights.holder |
Copyright: The American Society for Biochemistry and Molecular Biology, Inc. |
en |
dc.identifier.pmid |
16864584 |
en |
pubs.end-page |
29095 |
en |
dc.rights.accessrights |
http://purl.org/eprint/accessRights/RestrictedAccess |
en |
pubs.subtype |
Article |
en |
pubs.elements-id |
68325 |
en |
pubs.org-id |
Medical and Health Sciences |
en |
pubs.org-id |
Medical Sciences |
en |
pubs.org-id |
Molecular Medicine |
en |
pubs.org-id |
Pharmacology |
en |
pubs.org-id |
Science |
en |
pubs.org-id |
Biological Sciences |
en |
pubs.record-created-at-source-date |
2010-09-01 |
en |
pubs.dimensions-id |
16864584 |
en |