Induction of Krox-24 by endogenous cannabinoid type 1 receptors in Neuro2A cells is mediated by the MEK-ERK MAPK pathway and is suppressed by the phosphatidylinositol 3-kinase pathway.

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dc.contributor.author Graham, Euan en
dc.contributor.author Ball, N en
dc.contributor.author Scotter, Emma en
dc.contributor.author Narayan, Pritika en
dc.contributor.author Dragunow, Michael en
dc.contributor.author Glass, Michelle en
dc.date.accessioned 2012-05-30T04:20:17Z en
dc.date.issued 2006 en
dc.identifier.citation Journal of Biological Chemistry 281(39):29085-29095 2006 en
dc.identifier.issn 0021-9258 en
dc.identifier.uri http://hdl.handle.net/2292/18788 en
dc.description.abstract Neuro2a cells endogenously express cannabinoid type 1 (CB1) receptors. CB1 stimulation with HU210 activated ERK and induced the transcription factor Krox-24. A functional MEK-ERK pathway is an important requirement for CB1-mediated Krox-24 induction as blockade of MEK signaling by UO126 reduces both basal and CB1-mediated activation of Krox-24. CB1 receptor stimulation did not activate either JNK or p38 MAPK pathways or the pro-proliferation phosphatidylinositol 3-kinase (PI3K)-Akt pathway. However, serum removal or blockade of PI3K signaling by LY294002 transiently stimulated basal Krox-24 expression and increased CB1-mediated induction of Krox-24. This was consistent with a transient increase in pMEK, pERK, and pCREB levels following PI3K blockade. These data demonstrate that CB1-mediated activation of the Krox-24 transcription factor is negatively regulated through the PI3K-Akt pathway and reveals several points of signaling cross-talk between these two important kinase pathways. en
dc.publisher The American Society for Biochemistry and Molecular Biology, Inc. en
dc.relation.ispartofseries Journal of Biological Chemistry en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. Details obtained from http://www.sherpa.ac.uk/romeo/issn/0021-9258/ en
dc.rights.uri https://researchspace.auckland.ac.nz/docs/uoa-docs/rights.htm en
dc.title Induction of Krox-24 by endogenous cannabinoid type 1 receptors in Neuro2A cells is mediated by the MEK-ERK MAPK pathway and is suppressed by the phosphatidylinositol 3-kinase pathway. en
dc.type Journal Article en
dc.identifier.doi 10.1074/jbc.M602516200 en
pubs.issue 39 en
pubs.begin-page 29085 en
pubs.volume 281 en
dc.rights.holder Copyright: The American Society for Biochemistry and Molecular Biology, Inc. en
dc.identifier.pmid 16864584 en
pubs.end-page 29095 en
dc.rights.accessrights http://purl.org/eprint/accessRights/RestrictedAccess en
pubs.subtype Article en
pubs.elements-id 68325 en
pubs.org-id Medical and Health Sciences en
pubs.org-id Medical Sciences en
pubs.org-id Molecular Medicine en
pubs.org-id Pharmacology en
pubs.org-id Science en
pubs.org-id Biological Sciences en
pubs.record-created-at-source-date 2010-09-01 en
pubs.dimensions-id 16864584 en


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