In Silico Study on a New Orthologs SLC25A3 and It’s Experimental Verification in Porcine Ovary

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Show simple item record Zeng-xiang, PAN en Jin-bi, ZHANG en Fei, LIN en Qi-fa, LI en Zhuang, XIE en Hong-lin, LIU en 2012-06-26T22:38:49Z en 2010 en
dc.identifier.citation Scientia Agricultura Sinica 43:1926-1932 Article number 9 2010 en
dc.identifier.issn 0578-1752 en
dc.identifier.uri en
dc.description.abstract 【Objective】 The objective of this study is to efficiently obtain the structural and functional information of orthologs. 【Method】 The approach of gene-oriented ESTs annotation based on model organisms, in silico analysis and the method of primers extension were combined in the study.【Result】 An orthologs SLC25A3 (Solute Carrier Family 25, Member 3)by the approach of gene- oriented ESTs annotation based on human cDNA sequences was identified, and then the homologous ESTs expressed in porcine ovary were selected for in silico cloning and experimental verification, and ulmately the structural and functional information of the orthologs SLC25A3 were obtained by bioinformatic methods.【Conclusion】 Combining the gene-oriented ESTs annotation method and the relative bioinformatcic tools, the sequene and functional information of orthologs could be obtained fastly and directly, and the transformation or communication of the valuable information between different species could be realized, thus providing reliable chioces for sequence analysis and functional annotation in comparative genomic research. en
dc.publisher Chinese Electronic Periodical Services en
dc.relation.ispartofseries Scientia Agricultura Sinica en
dc.rights Items in ResearchSpace are protected by copyright, with all rights reserved, unless otherwise indicated. Previously published items are made available in accordance with the copyright policy of the publisher. en
dc.rights.uri en
dc.title In Silico Study on a New Orthologs SLC25A3 and It’s Experimental Verification in Porcine Ovary en
dc.type Journal Article en
dc.identifier.doi 10.3864/j.issn.0578-1752.2010.09.020 en
pubs.begin-page 1926 en
pubs.volume 43 en
dc.rights.holder Copyright: Chinese Electronic Periodical Services en
pubs.end-page 1932 en
dc.rights.accessrights en
pubs.subtype Article en
pubs.elements-id 343757 en
pubs.number 9 en
pubs.record-created-at-source-date 2012-04-18 en

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